Reference : Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progen...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/3709
Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4.
English
Wislet-Gendebien, Sabine mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Bruyere, Françoise mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Hans, Grégory mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
Leprince, Pierre mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Moonen, Gustave mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Rogister, Bernard mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
2004
BMC Neuroscience
BioMed Central
5
33
Yes (verified by ORBi)
International
1471-2202
England
[en] Animals ; Antigens, Differentiation/biosynthesis ; Astrocytes/cytology ; Bone Marrow Cells/cytology ; Bone Morphogenetic Proteins/biosynthesis/physiology/secretion ; Cell Count ; Cell Differentiation/drug effects/physiology ; Cell Division ; Cell Lineage ; Cells, Cultured ; Coculture Techniques ; Culture Media, Conditioned/pharmacology ; Cytokines/genetics ; Glial Fibrillary Acidic Protein/biosynthesis ; Intermediate Filament Proteins/biosynthesis ; Mesenchymal Stem Cell Transplantation/methods ; Mesenchymal Stem Cells/cytology/metabolism/secretion ; Mice ; Mice, Inbred Strains ; Nerve Tissue Proteins/biosynthesis ; Neurons/cytology ; RNA, Messenger/biosynthesis ; Rats ; Stem Cells/cytology/drug effects/metabolism
[en] BACKGROUND: Spontaneous repair is limited after CNS injury or degeneration because neurogenesis and axonal regrowth rarely occur in the adult brain. As a result, cell transplantation has raised much interest as potential treatment for patients with CNS lesions. Several types of cells have been considered as candidates for such cell transplantation and replacement therapies. Foetal brain tissue has already been shown to have significant effects in patients with Parkinson's disease. Clinical use of the foetal brain tissue is, however, limited by ethical and technical problems as it requires high numbers of grafted foetal cells and immunosuppression. Alternatively, several reports suggested that mesenchymal stem cells, isolated from adult bone marrow, are multipotent cells and could be used in autograft approach for replacement therapies. RESULTS: In this study, we addressed the question of the possible influence of mesenchymal stem cells on neural stem cell fate. We have previously reported that adult rat mesenchymal stem cells are able to express nestin in defined culture conditions (in the absence of serum and after 25 cell population doublings) and we report here that nestin-positive (but not nestin-negative) mesenchymal stem cells are able to favour the astroglial lineage in neural progenitors and stem cells cultivated from embryonic striatum. The increase of the number of GFAP-positive cells is associated with a significant decrease of the number of Tuj1- and O4-positive cells. Using quantitative RT-PCR, we demonstrate that mesenchymal stem cells express LIF, CNTF, BMP2 and BMP4 mRNAs, four cytokines known to play a role in astroglial fate decision. In this model, BMP4 is responsible for the astroglial stimulation and oligodendroglial inhibition, as 1) this cytokine is present in a biologically-active form only in nestin-positive mesenchymal stem cells conditioned medium and 2) anti-BMP4 antibodies inhibit the nestin-positive mesenchymal stem cells conditioned medium inducing effect on astrogliogenesis. CONCLUSIONS: When thinking carefully about mesenchymal stem cells as candidates for cellular therapy in neurological diseases, their effects on resident neural cell fate have to be considered.
Centre de neurobiologie cellulaire et moleculaire
Fonds National de la Recherche Scientifique (FNRS) of Belgium ; Fondation Médical Reine Elisabeth (FMRE) ; Fonds Charcot ; Belgian League against Multiple Sclerosis
Researchers
http://hdl.handle.net/2268/3709
10.1186/1471-2202-5-33

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