Reference : Microanalytical systems for separations of stratum corneum ceramides
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/36837
Microanalytical systems for separations of stratum corneum ceramides
English
Quinton, Loïc mailto [Université Paris-Sud 11 > Laboratoire de Chimie Analytique, Faculté de pharmacie, Châtenay-Malabry, France > > >]
Gaudin, Karen [Université Paris-Sud 11 > Laboratoire de Chimie Analytique, Faculté de pharmacie, Châtenay-Malabry, France > > >]
Baillet, Arlette [Université Paris-Sud 11 > Laboratoire de Chimie Analytique, Faculté de pharmacie, Châtenay-Malabry, France > > >]
Chaminade, Pierre mailto [Université Paris-Sud 11 > Laboratoire de Chimie Analytique, Faculté de pharmacie, Châtenay-Malabry, France > > >]
2006
Journal of Separation Science
Wiley-VCH Verlag Gmbh
29
3
390-398
Yes (verified by ORBi)
International
1615-9306
1615-9314
Weinheim
Germany
[en] ceramides ; Evaporative light scattering detector ; Polymerized vinyl alcohol phase ; stratum corneum ; Porous graphitic carbon
[en] The small amount of lipids from human skin obtained with noninvasive sampling
method led us to investigate microanalytical separation techniques. The lipid class
analysis was performed with a micro polyvinyl alcohol-silica (PVA-Silm) column. The
gradient elution was from heptane to acetone/butanol 90:10 v/v in 4%/min at 78 lL/
min. In addition an evaporative light scattering detector (ELSD) was modified for
micro-LC. All solvents contained 0.1% of triethylamine and formic acid in stoichiometric
amount, which increased the ELSD response. In these conditions, the cholesterol
eluted before free fatty acid, and squalene and triglycerides close to the dead
volume. The various ceramide classes eluted following the order of the increased
number of hydroxyl groups. The LOD for ceramides was 2.2 ng. The advantages of
this method are the use of a normal stationary phase more reliable due to its chemical
stability, its surface homogeneity and its development in microchromatography
without chlorinated solvents which offers small LOD and the whole profile of lipids
present in stratum corneum (SC). A method using a narrow-bore PVA-Sil column
was used to collect ceramide fraction. Then the molecular species were analysed
with a porous graphitic carbon column in capillary LC using a gradient from
CH3OH/CHCl3 70:30 v/v to CHCl3 at 2%/min with a flow rate at 5 lL/min. The LOD
obtained for ceramide was 1 ng. Both methods were assessed with SC samples obtained
by rinsing a 5.7 cm2 area of the forearm with 25 mL of ethanol.
http://hdl.handle.net/2268/36837
10.1002/jssc.200500362
http://www3.interscience.wiley.com/journal/112395382/abstract

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