Reference : Localization of varicella-zoster virus nucleic acids and proteins in human skin.
Scientific journals : Article
Life sciences : Microbiology
Human health sciences : Dermatology
Localization of varicella-zoster virus nucleic acids and proteins in human skin.
Nikkels, Arjen mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatologie >]
Debrus, S. [> > > >]
Sadzot-Delvaux, Catherine mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique >]
Piette, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research >]
Rentier, Bernard mailto [Université de Liège - ULg > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie >]
Pierard, Gérald mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
Lippincott Williams & Wilkins
12 Suppl 8
Yes (verified by ORBi)
[en] DNA, Viral/analysis ; Herpes Zoster/genetics/metabolism ; Herpesvirus 3, Human/genetics ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Skin/chemistry ; Viral Proteins/analysis
[en] The pathogenic mechanisms involved in varicella-zoster virus (VZV) infections remain elusive. The pattern of cutaneous distribution of the IE63 protein and of the gpI (gE) and gpII glycoproteins with their corresponding genome sequences during VZV infections was studied by immunohistochemistry and in situ hybridization. Skin biopsy specimens were obtained from immunocompetent and immunocompromised patients with varicella, herpes zoster, or atypical VZV lesions. The first evidence for VZV infection consisted of the presence of IE63 in keratinocytes. In the vesicles and pustules, the viral transcripts gpI, gpII, and IE63 and the corresponding nucleic acids for gpI and gpII were identified in keratinocytes, sebocytes, Langerhans cells, dermal dendrocytes, monocytes/macrophages, and endothelial cells. The gpI and gpII glycorpoteins were essentially located on the cellular membranes while IE63 expression was generally restricted to the nuclei. In three biopsies of early herpes zoster, viral proteins were disclosed in dermal nerves and in perineurial type I dendrocytes. This was never encountered in varicella. Vasculitic changes and endothelial cell involvement were more prominent in varicella than in herpes zoster. It is concluded that the secondary viremia in varicella that affects the dermal endothelial cells is followed by a cell-to-cell spread to keratinocytes. In herpes zoster, the viral progression through cutaneous nerves primarily extends to the pilosebaceous units with a secondary involvement of epidermal keratinocytes, followed by a further spread to dermal cells.

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