Reference : Immunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE...
Scientific journals : Article
Human health sciences : Dermatology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/36775
Immunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE63) and late (gE) protein on smears and cutaneous biopsies: implications for diagnostic use.
English
Nikkels, Arjen mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatologie >]
Debrus, S. [> > > >]
Sadzot-Delvaux, Catherine mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique >]
Piette, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research >]
Rentier, Bernard mailto [Université de Liège - ULg > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie >]
Pierard, Gérald mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
1995
Journal of Medical Virology
Wiley Liss, Inc.
47
4
342-7
Yes (verified by ORBi)
International
0146-6615
1096-9071
New York
NY
[en] Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antigens, Viral/immunology ; Chickenpox/immunology/pathology/virology ; Female ; Herpes Zoster/immunology/pathology/virology ; Herpesvirus 3, Human/immunology/isolation & purification ; Humans ; Immediate-Early Proteins/immunology ; Immunohistochemistry/methods ; Male ; Mice ; Middle Aged ; Rabbits ; Sensitivity and Specificity ; Skin/immunology/pathology/virology ; Swine ; Viral Envelope Proteins/immunology
[en] Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical and immunohistochemical identification of the VZV ORF63 encoded protein (IE63) and of the VZV late protein gE on smears and formalin-fixed paraffin-embedded skin sections taken from lesions clinically diagnosed as varicella (n = 15) and herpes zoster (n = 51). Microscopic examinations of Tzanck smears and skin sections yielded a diagnostic accuracy of Herpesviridae infections in 66.7% (10/15) and 92.3% (12/13) of varicella, and 74.4% (29/39) and 87.8% (43/49) of herpes zoster, respectively. Immunohistochemistry applied to varicella provided a type-specific virus diagnostic accuracy of 86.7% (13/15; IE63) and 100% (15/15; gE) on smears, and of 92.3% for both VZV proteins on skin sections. In herpes zoster, the diagnostic accuracy of immunohistochemistry reached 92.3% (36/39; IE63) and 94.9% (37/39; gE) on smears, and 91.7% (44/48; IE63) and 91.8% (45/49; gE) on skin sections. These findings indicate that the immunohistochemical detection of IE63 and gE on both smears and skin sections yields a higher specificity and sensitivity than standard microscopic assessments.
Researchers
http://hdl.handle.net/2268/36775

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