ORBi: Nikkels Arjen - Immunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE63) and late (gE) protein on smears and cutaneous biopsies: implications for diagnostic use.

Reference : Immunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Dermatology Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/36775

Title :	Immunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE63) and late (gE) protein on smears and cutaneous biopsies: implications for diagnostic use.
Language :	English
Author, co-author :	Nikkels, Arjen [Centre Hospitalier Universitaire de Liège - CHU > > Dermatologie >]
	Debrus, S. [> > > >]
	Sadzot-Delvaux, Catherine [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique >]
	Piette, Jacques [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research >]
	Rentier, Bernard [Université de Liège - ULg > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie >]
	Pierard, Gérald [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
Publication date :	1995
Journal title :	Journal of Medical Virology
Publisher :	Wiley Liss, Inc.
Volume :	47
Issue/season :	4
Pages :	342-7
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0146-6615
e-ISSN :	1096-9071
City :	New York
Country :	NY
Keywords :	[en] Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antigens, Viral/immunology ; Chickenpox/immunology/pathology/virology ; Female ; Herpes Zoster/immunology/pathology/virology ; Herpesvirus 3, Human/immunology/isolation & purification ; Humans ; Immediate-Early Proteins/immunology ; Immunohistochemistry/methods ; Male ; Mice ; Middle Aged ; Rabbits ; Sensitivity and Specificity ; Skin/immunology/pathology/virology ; Swine ; Viral Envelope Proteins/immunology
Abstract :	[en] Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical and immunohistochemical identification of the VZV ORF63 encoded protein (IE63) and of the VZV late protein gE on smears and formalin-fixed paraffin-embedded skin sections taken from lesions clinically diagnosed as varicella (n = 15) and herpes zoster (n = 51). Microscopic examinations of Tzanck smears and skin sections yielded a diagnostic accuracy of Herpesviridae infections in 66.7% (10/15) and 92.3% (12/13) of varicella, and 74.4% (29/39) and 87.8% (43/49) of herpes zoster, respectively. Immunohistochemistry applied to varicella provided a type-specific virus diagnostic accuracy of 86.7% (13/15; IE63) and 100% (15/15; gE) on smears, and of 92.3% for both VZV proteins on skin sections. In herpes zoster, the diagnostic accuracy of immunohistochemistry reached 92.3% (36/39; IE63) and 94.9% (37/39; gE) on smears, and 91.7% (44/48; IE63) and 91.8% (45/49; gE) on skin sections. These findings indicate that the immunohistochemical detection of IE63 and gE on both smears and skin sections yields a higher specificity and sensitivity than standard microscopic assessments.
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/36775

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