chlorophyllide; etioplasts; fluorescence spectroscopy; Photosystem II assembly; protochlorophyllide; variable fluorescence
Abstract :
[en] Chlorophyll(ide) spectroscopic properties and Photosystem II assembly, monitored by 77 K variable fluorescence, were studied in etiolated barley leaves as a function of the extent of protochlorophyllide photoreduction by a single millisecond light flash of different intensities. Variable fluorescence, measured 2 hours after the flash, was only detected when the extent of phototransformation was higher than a threshold value of 0.4. Its development paralleled the formation of a chlorophyll emission component at 685 nm, which itself derived from long-wavelength chlorophyllide with an emission maximum at 695 nm. At low flash intensities, short-wavelength chlorophyllide forms preferentially accumulated and no Photosystem II fluorescence was detected after 2 hours. Chlorophyllide esterification was independent of the extent of phototransformation. These results suggested that the formation of long-wavelength chlorophyllide was essential for further assembly of Photosystem II. This interpretation was strengthened by the observed inhibition of both long-wavelength chlorophyllide formation and of variable fluorescence development in leaves treated with 6-aminolevulinic acid or in untreated leaves subjected to repeated flashes of low intensity.
Böddi B, Ryberg M and Sundqvist C (1991) The formation of a short-wavelength chlorophyllide form at partial phototransformation of protochlorophyllide in etioplast inner membranes. Photochem Photobiol 53: 667-673
Eichacker LA, Soll J, Lauterbach P, Rüdiger W, Klein RR and Mullet JE (1990) In vitro synthesis of chlorophyll a in the dark triggers accumulation of chlorophyll a apo-proteins in barley etioplasts. J Biol Chem. 265: 13566-13571
El Hamouri B, Brouers M and Sironval C (1981) Pathway from photoactive P633-628 protochlorophyllide to the P696-682 chlorophyllide in cucumber etioplast suspensions Plant Sci Lett 21: 375-379
Franck F (1993a) Photosynthetic activities during early assembly of thylakoid membranes. In: Ryberg M and Sundqvist C (eds) Pigment-Protein Complexes in Plastids: Synthesis and Assembly, pp 365-381. Academic Press, San Diego, CA
Franck F (1993b) On the formation of Photosystem II chlorophyll-proteins after a short flash in etiolated barley leaves, as monitored by in vivo fluorescence spectroscopy. J Photochem Photobiol B 18: 35-40
Gassman M (1973) The conversion of photoinactive protochlorophyllide633 to phototransformable protochlorophyllide650 in etiolated bean leaves treated with δ-aminolevulinic acid. Plant Physiol 52: 590-594
Glick RE and Melis A (1988) Minimum photosynthetic unit size in system I and system II of barley chloroplasts. Biochim Biophys Acta 934: 151-155
Granick S and Gassman M (1970) Rapid regeneration of protochlorophyllide 650. Plant Physiol 45: 201-205
Henningsen KW (1970) Macromolecular physiology of plastids VI. Changes in membrane structure associated with shifts in the absorption maxima of the chlorophyllous pigments. J Cell Sci 7: 587-621
Henningsen KW and Thorne SW (1974) Esterification and spectral shifts of chlorophyll(ide) in wild-type and mutant seedlings developed in darkness. Physiol Plant 30: 82-89
Holtorf H, Reinbothe S, Reinbothe C, Bereza B and Apel K (1995) Two routes of chlorophyllide synthesis that are differentially regulated by light in barley. Proc Natl Acad Sci USA 92: 3254-3258
Ignatov NV and Litvin FF (1994) Photoinduced formation of pheophytin/chlorophyll-containing complexes during greening of plant leaves. Photosynth Res 42: 27-35
Kahn A, Boardman NK and Thorne SW (1970) Energy transfer between protochlorophyllide molecules: Evidence for multiple chromophores in the photoactive protochlorophyllide-protein complex in vivo and in vitro. J Mol Biol 48: 85-101
Klein RR, Gamble PE and Mullet JE (1988) Light-dependent accumulation of radiolabeled plastid-encoded chlorophyll-a apoproteins requires chlorophyll a: I. Analysis of chlorophyll-deficient mutants and phytochrome involvement. Plant Physiol 88: 1246-1256
Klein S, Bryan G and Bogorad L (1964) Early stages in the development of plastid fine structure in red and far-red light. J Cell Sci 22: 433-442
Klein S and Bogorad L (1964) Fine structural changes in proplastids during photodestruction of pigments. J Cell Sci 22: 443-451
Lebedev N, van Cleve B, Armstrong G and Apel K (1995) Chlorophyll synthesis in a deetiolated (det340) mutant of Arabidopsis without NADPH-protochlorophyllide (PChlide) oxidoreductase (POR) A and photoactive PChlide-F655. Plant Cell 7: 2081-2090
Lindsten A, Welsch CJ, Schoch S, Ryberg M, Rüdiger W and Sundqvist C (1990) Chlorophyll synthetase is latent in well preserved prolamellar bodies of etiolated wheat. Physiol Plant 80: 277-285
McCormac DJ, Marwood CA, Bruce D and Greenberg BM (1996) Assembly of Photosystem I and II during the early phases of light-induced development of chloroplasts from proplastids in Spirodela oligorrhiza. Photochem Photobiol: 63: 837-845
Michel JM and Sironval C (1973) Effet de la fréquence d'éclairs de faible intensité sur les caractères des changements spectraux produits dans une feuille étiolée. Physiol Vég 11: 291-300
Michel JM and Sironval C (1977) Shifts to C675-S670 and to C696-S684 in etiolated leaves illuminated with series of brief flashes. Plant Cell Physiol 18: 1223-1234
Oliver RP and Griffiths WT (1982) Pigment-protein complexes of illuminated etiolated leaves. Plant Physiol 70: 1019-1025
Rüdiger W, Benz J and Guthoff C (1980) Detection and partial characterization of activity of chlorophyll synthetase in etioplast membranes. Eur J Biochem. 109: 193-200
Ryberg M and Sundqvist C (1982) Spectral forms of protochlorophyllide in prolamellar bodies and prothylakoids fractionated from wheat etioplasts. Physiol Plant 56: 133-138
Ryberg M, Artus N, Böddi B, Lindsten A, Wiktorsson B and Sundqvist C (1992) Pigment-protein complexes of chlorophyll precursors. In: Argyroudi-Akoyunoglou JH (ed) Regulation of Chloroplast Biogenesis, pp 217-224. Plenum Press, New York
Schoefs B and Franck F (1993) Photoreduction of protochlorophyllide to chlorophyllide in 2-d-old dark-grown bean (Phaseolus vulgaris cv. Commodore) leaves. J Exp Bot 44: 1053-1057
Shibata K (1957) Spectroscopic studies on chlorophyll formation in intact leaves. J Biochem 44: 147-173
Sigrist M and Staehelin LA (1994) Appearance of type 1, 2, and 3 light-harvesting complex II and light-harvesting complex I proteins during light-induced greening of barley (Hordeum vulgare) etioplasts. Plant Physiol 104: 135-145
Sironval C and Brouers M (1980) The reduction of protochlorophyllide. VIII. The theory of transfer units. Photosynthetica 14: 213-221
Sironval C, Michel-Wolwertz MR and Madsen A (1965) On the nature and possible functions of the 673- and 684 m forms in vivo of chlorophyll. Biochim Biophys Acta 94: 344-354
Smith JHC (1954) The development of chlorophyll and oxygen-evolving power in etiolated barley leaves when illuminated. Plant Physiol 29: 143-148
Sundqvist C (1969) Transformation of protochlorophyllide, formed from exogenous δ-amino-levulinic acid, in continuous light and in flash light. Physiol Plant 22: 147-156
Virgin HI, Kahn A and von Wettstein D (1963) The physiology of chlorophyll formation in relation to structural changes of chloroplasts. Photochem. Photobiol 2: 83-91
von Wettstein D, Gough S and Kannangara CG (1995) Chlorophyll Biosynthesis. Plant Cell 7: 1039-1057
Wellburn AR and Hampp R (1979) Appearance of photochemical function in prothylakoids during plastid development. Biochim Biophys Acta 547: 380-394
White MJ and Green BR (1988) Intermittent-light chloroplasts are not developmentally equivalent to chlorina f2 chloroplasts in barley. Photosynth Res 15: 195-203