Article (Scientific journals)
Formation of long-wavelength chlorophyllide (Chlide695) is required for the assembly of Photosystem II in etiolated barley leaves
Franck, Fabrice; Eullaffroy, P.; Popovic, R.
1997In Photosynthesis Research, 51 (2), p. 107-118
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Keywords :
chlorophyllide; etioplasts; fluorescence spectroscopy; Photosystem II assembly; protochlorophyllide; variable fluorescence
Abstract :
[en] Chlorophyll(ide) spectroscopic properties and Photosystem II assembly, monitored by 77 K variable fluorescence, were studied in etiolated barley leaves as a function of the extent of protochlorophyllide photoreduction by a single millisecond light flash of different intensities. Variable fluorescence, measured 2 hours after the flash, was only detected when the extent of phototransformation was higher than a threshold value of 0.4. Its development paralleled the formation of a chlorophyll emission component at 685 nm, which itself derived from long-wavelength chlorophyllide with an emission maximum at 695 nm. At low flash intensities, short-wavelength chlorophyllide forms preferentially accumulated and no Photosystem II fluorescence was detected after 2 hours. Chlorophyllide esterification was independent of the extent of phototransformation. These results suggested that the formation of long-wavelength chlorophyllide was essential for further assembly of Photosystem II. This interpretation was strengthened by the observed inhibition of both long-wavelength chlorophyllide formation and of variable fluorescence development in leaves treated with 6-aminolevulinic acid or in untreated leaves subjected to repeated flashes of low intensity.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Franck, Fabrice ;  Université de Liège - ULiège > Biochimie végétale
Eullaffroy, P.
Popovic, R.
Language :
English
Title :
Formation of long-wavelength chlorophyllide (Chlide695) is required for the assembly of Photosystem II in etiolated barley leaves
Publication date :
1997
Journal title :
Photosynthesis Research
ISSN :
0166-8595
eISSN :
1573-5079
Publisher :
Springer, Dordrecht, Netherlands
Volume :
51
Issue :
2
Pages :
107-118
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 May 2010

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