ORBi: Sperling U. - Etioplast differentiation in Arabidopsis: Both PORA and PORB restore the prolamellar body and photoactive protochlorophyllide-F655 to the cop1 photomorphogenic mutant

Reference : Etioplast differentiation in Arabidopsis: Both PORA and PORB restore the prolamellar bod...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...) Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/36610

Title :	Etioplast differentiation in Arabidopsis: Both PORA and PORB restore the prolamellar body and photoactive protochlorophyllide-F655 to the cop1 photomorphogenic mutant
Language :	English
Author, co-author :	Sperling, U. [> > > >]
	Franck, Fabrice [Université de Liège - ULg > > Biochimie végétale >]
	van Cleve, B. [> > > >]
	Frick, G. [> > > >]
	Apel, K. [> > > >]
	Armstrong, G. A. [> > > >]
Publication date :	1998
Journal title :	Plant Cell
Publisher :	American Society of Plant Biologists
Volume :	10
Issue/season :	2
Pages :	283-296
Audience :	International
ISSN :	1040-4651
e-ISSN :	1532-298X
City :	Rockville
Country :	MD
Abstract :	[en] The etioplast plastid type of dark-grown angiosperms is defined by the accumulation of the chlorophyll (Chi) precursor protochlorophyllide (Pchlide) and the presence of the paracrystalline prolamellar body (PLB) membrane. Both features correlate with the presence of NADFH:Pchlide oxidoreductase (FOR), a light-dependent enzyme that reduces photoactive Pchlide-F655 to chlorophyllide and plays a key role in chloroplast differentiation during greening. Two differentially expressed and regulated FOR enzymes, FORA and FORB, have recently been discovered in angiosperms. To investigate the hypothesis that etioplast differentiation requires FORA, we have constitutively overexpressed PORA and FORB in the Arabidopsis wild type and in the constitutive photomorphogenic cop1-18 (previously det340) mutant, which is deficient in the PLB and Pchlide-F655. In both genetic backgrounds, FOR overexpression increased PLB size, the ratio of Pchlide-F655 to nonphotoactive Pchl[ide]-F632, and the amount of Pchlide-F655. Dramatically, restoration of either FORA or FORB to the cop1 mutant led to the formation of etioplasts containing an extensive PLB and large amounts of photoactive Pchlide-F655.
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/36610

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