ORBi: Thellin Olivier - Housekeeping Genes as Internal Standards: Use and Limits

Reference : Housekeeping Genes as Internal Standards: Use and Limits


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/3661

Title :	Housekeeping Genes as Internal Standards: Use and Limits
Language :	English
Author, co-author :	Thellin, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Zorzi, Willy [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Lakaye, Bernard [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	De Borman, B. [> > > >]
	Coumans, Bernard [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
	Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
	Grisar, Thierry [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique]
	Igout, Ahmed [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Publication date :	1999
Journal title :	Journal of Biotechnology
Publisher :	Elsevier Science
Volume :	75
Issue/season :	2-3
Pages :	291-5
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0168-1656
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] Amplification ; RNA ; PCR
Abstract :	[en] Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins, cyclophilin, hypoxantine phsophoribosyltransferase (HRPT), L32. 28S, and 18S rRNAs are also used as internal standards. In this paper, it is recalled that the commonly used internal standards can quantitatively vary in response to various factors. Possible variations are illustrated using three experimental examples. Preferred types of internal standards are then proposed for each of these samples and thereafter the general procedure concerning the choice of an internal standard and the way to manage its uses are discussed.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/3661
	also: http://hdl.handle.net/2268/23183

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