Article (Scientific journals)
Detection of the Photoactive Protochlorophyllide-Protein Complex in the Light During the Greening of Barley
Franck, Fabrice; Strzalka, K.
1992In FEBS Letters, 309 (1), p. 73-7
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Abstract :
[en] A photoactive protochlorophyllide-protein complex with absorbance and fluorescence maxima at 648 and 653 nm was detected in greening barley leaves without any re-darkening. The variations of the amplitudes of the absorbance and the fluorescence of the photoactive protochlorophyllide with greening time at two different light intensities indicate a close relationship between the rate of chlorophyll synthesis and the amount of the complex during the first hours. The chlorophyllide resulting from photoreduction during greening has an absorbance maximum at 684 nm, which shifts towards a shorter wavelength within a few seconds, indicating rapid liberation of the pigment from the enzyme. We conclude that chlorophyll accumulation proceeds through continuous regeneration and phototransformation of the photoactive complex.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Franck, Fabrice ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale
Strzalka, K.
Language :
English
Title :
Detection of the Photoactive Protochlorophyllide-Protein Complex in the Light During the Greening of Barley
Publication date :
31 August 1992
Journal title :
FEBS Letters
ISSN :
0014-5793
eISSN :
1873-3468
Publisher :
Wiley-Blackwell, United States
Volume :
309
Issue :
1
Pages :
73-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 May 2010

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