Localization of Nadph-Protochlorophyllide Reductase in Plastids of Barley at Different Greening Stages

[en] The localization of protochorophyllide (Pchlide) and of NADPH-protochlorophyllide oxidoreductase (POR, EC 1.6.99.1) within (etio)chloroplasts has been investigated at selected stages of greening of barley seedlings. Pchlide pigment and POR protein contents were evaluated in different plastid membrane fractions by fluorescence spectroscopy and immunoblot analysis using a monospecific polyclonal antibody raised against the purified enzyme. Fluorescence analysis showed the presence of Pchlide in both the envelope and thylakoid membranes. During greening, the Pchlide content, expressed on a total protein basis, decreased in thylakoid membranes, whereas it increased in the envelope membranes. POR proteins were detected mainly in thylakoid membranes at early greening stages. In contrast, the weak amount of POR proteins was associated more specifically with envelope membranes of mature chloroplasts. Whatever the greening stage, thylakoid-bound Pchlide and POR proteins were more abundant in the thylakoid regions which remained unsolubilized after mild Triton treatment used as standard procedure to prepare PS II particles. This suggests the preferential association of Pchlide and POR to the appressed regions of thylakoids.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Barthelemy, X.

Bouvier, G.

Radunz, A.

De Buyl, Simone

Schmid, G. H.

Franck, Fabrice ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale

Language :

English

Title :

Localization of Nadph-Protochlorophyllide Reductase in Plastids of Barley at Different Greening Stages

Publication date :

2000

Journal title :

Photosynthesis Research

ISSN :

0166-8595

eISSN :

1573-5079

Publisher :

Kluwer Academic Publishers, Netherlands

Volume :

Issue :

Pages :

63-76

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 May 2010

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Bibliography

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