Reference : Validation of a method for the quantitation of ghrelin and unacylated ghrelin by HPLC.
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/3644
Validation of a method for the quantitation of ghrelin and unacylated ghrelin by HPLC.
English
Rozet, Eric mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Staes, Edith [> >]
Ucakar, Bernard [> > > >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Preat, Veronique [> > > >]
2010
Journal of Pharmaceutical & Biomedical Analysis
51
3
633-9
Yes (verified by ORBi)
International
0731-7085
1873-264X
England
[en] An HPLC/UV method was first optimized for the separation and quantitation of human acylated and unacylated (or des-acyl) ghrelin from aqueous solutions. This method was validated by an original approach using accuracy profiles based on tolerance intervals for the total error measurement. The concentration range that achieved adequate accuracy extended from 1.85 to 59.30microM and 1.93 to 61.60microM for acylated and unacylated ghrelin, respectively. Then, optimal temperature, pH and buffer for sample storage were determined. Unacylated ghrelin was found to be stable in all conditions tested. At 37 degrees C acylated ghrelin was stable at pH 4 but unstable at pH 7.4, the main degradation product was unacylated ghrelin. Finally, this validated HPLC/UV method was used to evaluate the binding of acylated and unacylated ghrelin to liposomes.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/3644
10.1016/j.jpba.2009.09.040

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