Article (Scientific journals)
A multistage process to enhance cellobiose production from cellulosic materials
Vanderghem, Caroline; Boquel, Pascal; Blecker, Christophe et al.
2010In Applied Biochemistry and Biotechnology, 160 (8), p. 2300-2307
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Abstract :
[en] Cellobiose, a disaccharide, is a valuable product that can be obtained from cellulose hydrolysis. In this study, a simple methodology is presented to enhance the production and improve the selectivity of cellobiose during enzymatic hydrolysis of cellulose. The approach consisted of a multistage removal of filtrate via vacuum filtration and resuspension of the retentate. By this process, the remaining solid was further hydrolyzed without additional enzyme loading. Compared to the continuous hydrolysis process, the production of cellobiose increased by 45%. Increased selectivity of cellobiose is due to the loss of beta-glucosidases in the filtrate, while enhanced productivity is likely due to mitigated product inhibition.
Disciplines :
Food science
Author, co-author :
Vanderghem, Caroline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Boquel, Pascal
Blecker, Christophe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Paquot, Michel ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Language :
English
Title :
A multistage process to enhance cellobiose production from cellulosic materials
Publication date :
2010
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press, Totowa, United States - New Jersey
Volume :
160
Issue :
8
Pages :
2300-2307
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 May 2010

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