Article (Scientific journals)
Influence of culture conditions on lipopeptide production by Bacillus subtilis
Akpa, E.; Jacques, Philippe; Wathelet, Bernard et al.
2001In Applied Biochemistry and Biotechnology, 91-3, p. 551-561
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Abstract :
[en] Bacillus subtilis produces various families of lipopeptides with different homologous compounds. To produce "new molecules" with improved activities and to select strains that produced a reduced number of homologs or isomers, we studied the effects of different media on the nature of the synthesis of fatty; acid chains for each lipopeptide family. This study focused on two B. subtilis strains cultivated in flasks. Optimized medium for lipopeptide production and Landy medium modified by replacing glutamic acid with other alpha -amino acids were used. We found that the intensity of production of homologous compounds depends on the strain and the culture medium. Analysis of these lipopeptides by high-performance liquid chromatography showed that the strain R. subtilis NT02 yielded various homologous compounds when cultivated in Landy medium (L-Glu), but primarily one homologous product in hi,oh relative amounts when cultivated in the optimized medium. Mass spectrometric analysis and determination of the amino acid composition of this molecule enabled us to identify it as Bacillomycine L c15.
Disciplines :
Agriculture & agronomy
Author, co-author :
Akpa, E.
Jacques, Philippe 
Wathelet, Bernard ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Paquot, Michel ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Fuchs, R.
Budzikiewicz, H.
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles
Language :
English
Title :
Influence of culture conditions on lipopeptide production by Bacillus subtilis
Publication date :
2001
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press, Totowa, United States - New Jersey
Volume :
91-3
Pages :
551-561
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 May 2010

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