ORBi: Fett Thomas - Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins

Reference : Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/3589

Title :	Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins
Language :	English
Author, co-author :	Fett, Thomas [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
	Zecchinon, Laurent [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
	Vanden Bergh, Philippe [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
	Desmecht, Daniel [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Publication date :	2008
Journal title :	Microbial Pathogenesis
Publisher :	Academic Press
Volume :	45
Pages :	337-342
Audience :	International
ISSN :	0882-4010
City :	London
Country :	United Kingdom
Keywords :	[en] Caprine ; Mannheimia haemolytica
Abstract :	[en] Mannheimiosis is the major respiratory disease among some ruminants, whereas it is not pathogenic for other mammals, an observation that has been attributed to a specific interaction between Mannheimia haemolytica leukotoxin (Lkt) and bovine or ovine CD18 subunit of lymphocyte function-associated antigen-1 (LFA-1) and Mac-1. We therefore hypothesized that Lkt utilizes CD18 as its receptor on caprine leukocytes as well. We have transiently transfected the b2-integrins-deficient K-562 cell line with cDNAs encoding caprine CD11a and caprine CD18 to determine the susceptibility of the transfectants to Lktinduced cytolysis. Flow cytometric analysis of the transfectants revealed surface expression of caprine LFA-1 and lysis by Lkt in a concentration-dependent manner whereas the parent cells were not. Moreover, K562 cells expressing caprine CD18 and human or bovine CD11a were also sensitive to Lkt whereas K-562 cells expressing caprine CD11a and human CD18 were not. Taken together, these results indicate that CD18 on caprine leukocytes serves as a receptor for Lkt.
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/3589

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