Reference : Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human prom...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Physical, chemical, mathematical & earth Sciences : Physics
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/23125
Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1)
English
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Deby, Ginette [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Dogné, Jean-Michel [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
de Leval, X. [> > > >]
Kohnen, Stephan [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Navet, Rachel [Université de Liège - ULg > > GIGA - Formation >]
Sluse, Francis [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Hoebeke, Maryse [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Pirotte, Bernard mailto [Université de Liège - ULg > Département de pharmacie > Chimie pharmaceutique >]
Lamy, Maurice mailto [Université de Liège - ULg > Département des sciences cliniques > Anesthésie et réanimation]
2004
Biochemical and Biophysical Research Communications
Academic Press
325
4
1122-1130
Yes (verified by ORBi)
International
0006-291X
1090-2104
San Diego
CA
[en] Chlamydia pneumoniae ; cyclooxygenase-2 enzyme ; NADPH-oxidase ; COX-2 inhibitors ; ROS ; electron paramagnetic resonance ; chemiluminescence ; fluorescence ; oxymetry
[en] Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within atherosclerotic lesions. Reactive oxygen species produced by NADPH oxidase were found to trigger the cyclooxygenase-2 expression. The effects of preferential COX-2 inhibitors on ROS produced by Chlamydia-primed human monocytes (THP-1 cells) were evaluated by fluorescence, chemiluminescence, oxymetry, and EPR spin trapping. Fluorescence assays showed an increased production of ROS with Chlamydia versus cells primed by 10(-8) M PMA. COX-2 inhibitors inhibited in a dose-dependent manner the luminol-enhanced CL while ibuprofen and diclofenac increased the chemiluminescence response. By EPR spin trapping, COX-2 inhibitors, ibuprofen, and diclofenac, exhibited a dose-dependent inhibiting effect (10 and 100 muM) on the EPR signal appearance. Our cell model combining EPR, chemiluminescence, and oxymetry appeared relevant to study the modulating effects of preferential COX-2 inhibitors on the cell oxidant activity and chronic inflammatory diseases. (C) 2004 Elsevier Inc. All rights reserved.
Researchers ; Professionals
http://hdl.handle.net/2268/23125
also: http://hdl.handle.net/2268/861 ; http://hdl.handle.net/2268/35817
10.1016/j.bbrc.2004.10.155

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