Reference : Synoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenat...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/35812
Synoviocytes, not chondrocytes, release free radicals after cycles of anoxia/re-oxygenation
English
Schneider, Nicole mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Lejeune, Jean-Philippe mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Deby-Dupont, Ginette P. [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Hoebeke, Maryse mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
26-Aug-2005
Biochemical & Biophysical Research Communications
Academic Press Inc Elsevier Science
334
2
669-673
Yes (verified by ORBi)
International
0006-291X
San Diego
[en] equine ; articular chondrocytes ; synoviocytes ; free radical ; electron spin resonance ; anoxia
[en] By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10(7)cells; O(2) consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n=3 for each cell line). Both types of synoviocytes showed a high O(2) consumption, which was slowered after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O(2) consumption, unchanged by anoxia, and no free radical production. These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
Centre de l’Oxygène, Recherche et Développement - CORD, Centre Européen du Cheval de Mont-le-Soie
Ministère wallon de l'agriculture, de la ruralité, de l'environnement et du tourisme
Researchers ; Professionals
http://hdl.handle.net/2268/35812
10.1016/j.bbrc.2005.06.147

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