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Fibronectin promotes rat Schwann cell growth and motility
Baron-Van Evercooren, Anne; Kleinman, Hinda K.; Seppa, H. E. et al.
1982In Journal of Cell Biology, 93 (1), p. 211-216
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Keywords :
Schwann cells; Fibronectin; Cell mobility
Abstract :
[en] Techniques are now available for culturing well characterized and purified Schwann cells. Therefore, we investigated the role of fibronectin in the adhesion, growth, and migration of cultured rat Schwann cells. Double-immunolabeling shows that, in primary cultures of rat sciatic nerve, Schwann cells (90%) rarely express fibronectin, whereas fibroblasts (10%) exhibit a granular cytoplasmic and fibrillar surface-associated fibronectin. Secondary cultures of purified Schwann cells do not express fibronectin. Exogenous fibronectin has a small effect on promoting the adhesion of Schwann cells to the substrate and does not significantly affect cell morphology, but it produced a surface fibrillar network on fibronectin on the secondary Schwann cells. Tritiated thymidine autoradiography revealed that addition of fibronectin to the medium, even at low concentrations, markedly stimulates Schwann cell proliferation, in both primary and secondary cultures. In addition, when cell migration was measured in a Boyden chamber assay, fibronectin was found to moderately, but clearly, stimulate directed migration or chemotaxis.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Baron-Van Evercooren, Anne;  National Institutes of Health - NIH - Bethesda, MD
Kleinman, Hinda K.;  National Institutes of Health - NIH - Bethesda, MD
Seppa, H. E.
Rentier, Bernard  ;  Université de Liège - ULiège > Laboratoire de Microbiologie générale et médicale
Dubois-Dalcq, Monique;  National Institutes of Health - NIH - Bethesda, MD
Language :
English
Title :
Fibronectin promotes rat Schwann cell growth and motility
Publication date :
1982
Journal title :
Journal of Cell Biology
ISSN :
0021-9525
eISSN :
1540-8140
Publisher :
Rockefeller University Press
Volume :
93
Issue :
1
Pages :
211-216
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 January 2009

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