Reference : Impact of a mutation in the mitochondrial LSU rRNA gene from Chlamydomonas reinhardti...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/35253
Impact of a mutation in the mitochondrial LSU rRNA gene from Chlamydomonas reinhardtii on the activity and the assembly of respiratory-chain complexes
English
Remacle, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
Gloire, Geoffrey mailto [Université de Liège - ULg > > Virologie - Immunologie >]
Cardol, Pierre mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie végétale >]
Matagne, René-Fernand mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
May-2004
Current Genetics
Springer-Verlag
45
5
323-330
Yes (verified by ORBi)
International
0172-8083
New York
[en] mutation ; ribosomal RNA ; GTPase domain ; Chlamydomonas reinhardtii ; mitochondria ; respiratory complexes
[en] Two substitutions A1090G and A1098C (together called the m mutation) located in the conserved GTPase domain of the mitochondrial LSU rRNA gene were recently shown to weakly compensate for the phenotypical effect of a -1T frameshift mutation in the mitochondrial cox1 gene of C. reinhardtii. In order to analyze the impact of the m mutation on the mitochondrial translational machinery, a strain carrying the m mutation but wild-type for the cox1 gene was isolated. We found that the growth and the respiratory rate of the m mutant were affected and that the activities of complexes I, III, and IV, all containing mitochondria-encoded subunits, were lowered. In contrast the activities of complex II and of the alternative oxidase, both encoded exclusively by the nuclear genome, were not modified. The steady-state levels of complex I enzyme and of several components of the respiratory complexes I, III, and IV were also reduced in the mutant. We moreover showed that m did not suppress other frameshift or UGA stop mutations which affect mitochondrial genes.
http://hdl.handle.net/2268/35253

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