Reference : Étude microscopique de la maturation de l’ovocyte équin dans le follicule quaternaire
Dissertations and theses : Master of advanced studies dissertation
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/3525
Étude microscopique de la maturation de l’ovocyte équin dans le follicule quaternaire
French
[fr] Microscopic study of equine oocyte maturation inside quaternary follicle
Ponthier, Jérôme mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
12-Sep-2007
Université de Liège
Diplôme d'étude approfondies en Sciences Vétérinaire option Médecine Vétérinaire
Beckers, Jean-François mailto
Balligand, Marc mailto
Ectors, Fabien mailto
Touati, Kamal mailto
Hanzen, Christian mailto
Antoine, Nadine mailto
[en] Horse ; Oocyte ; Microscopy
[en] In the mare, oocytes in vitro maturation protocols are still in development. To our knowledge, immature oocyte aspect under light microscopy has not been studied and compared with mature oocyte aspect. The aim of the study is to give a general description of immature oocytes and the relationships between the different aspects.
Follicles from ovaries obtained at slaughterhouse are punctured after measurement of their diameter. Different characters, such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy.
No correlation between oestral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. Thickness of zona pellucida differs between oocytes with polar or non polar ooplasm, but no other parameter studied showed an influence on oocyte diameter. No correlation was observed between follicle diameter and oocyte diameter.
Results show that no character observed under light microscopy is related to oocyte follicular origin or to any other character. Further studies have to be done to compare these oocytes with mature oocytes and to find the ultra-structural origin of characters observed under light microscopy.
Reproduction des carnivores domestiques et des équidés
Researchers
http://hdl.handle.net/2268/3525

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