Reference : Étude microscopique de la maturation de l’ovocyte équin dans le follicule quaternaire

	Document type :	Dissertations and theses : Master of advanced studies dissertation
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/3525

Title :	Étude microscopique de la maturation de l’ovocyte équin dans le follicule quaternaire
Language :	French
Alternative title :	[fr] Microscopic study of equine oocyte maturation inside quaternary follicle
Author, co-author :	Ponthier, Jérôme [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
Publication date :	12-Sep-2007
Institution :	Université de Liège
Name of the degree :	Diplôme d'étude approfondies en Sciences Vétérinaire option Médecine Vétérinaire
Superviser :	Beckers, Jean-François
President of the jury :	Balligand, Marc
Member of the jury :	Ectors, Fabien
	Touati, Kamal
	Hanzen, Christian
	Antoine, Nadine
Keywords :	[en] Horse ; Oocyte ; Microscopy
Abstract :	[en] In the mare, oocytes in vitro maturation protocols are still in development. To our knowledge, immature oocyte aspect under light microscopy has not been studied and compared with mature oocyte aspect. The aim of the study is to give a general description of immature oocytes and the relationships between the different aspects. Follicles from ovaries obtained at slaughterhouse are punctured after measurement of their diameter. Different characters, such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy. No correlation between oestral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. Thickness of zona pellucida differs between oocytes with polar or non polar ooplasm, but no other parameter studied showed an influence on oocyte diameter. No correlation was observed between follicle diameter and oocyte diameter. Results show that no character observed under light microscopy is related to oocyte follicular origin or to any other character. Further studies have to be done to compare these oocytes with mature oocytes and to find the ultra-structural origin of characters observed under light microscopy.
Research units :	Reproduction des carnivores domestiques et des équidés
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/3525

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Restricted access Mémoire DEA Ovocyte Jérôme PONTHIER.pdf Publisher postprint 1.29 MB Request copy

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