The OLFR1 gene encoding the HGMP07E putative olfactory receptor maps to the 17p12-17p13 region of the human genome and reveals a MspI restriction fragment lenght polymorphism
OLFR1 gene; putative olfactory receptor; human genome; polymorphism
Abstract :
[en] Olfactory receptors are believed to be encoded by an extremely large subfamily of G protein-coupled receptors. A human gene (OLFR1) encoding a member of this family was cloned from a genomic library by cross-hybridization with a gene fragment obtained by the polymerase chain reaction. The nucleotide sequence of a 3.4-kb EcoRI fragment was determined, and the protein sequence was deduced from the single open reading frame. The gene was assigned by in situ hybridization of metaphase chromosomes to the 17p13-->p12 region of the human genome, in proximity to the tumor-suppressor gene encoding p53. When used as a probe on Southern blots under moderately stringent conditions, it hybridizes to at least three closely related genes. A restriction fragment length polymorphism was detected after MspI digestion. Mendelian segregation of the gene was assessed in three CEPH families, and linkage analysis confirmed the localization of the locus
Schurmans, Stéphane ; Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Institut de Biologie et de Médecine Moléculaire
Muscatelli, F.; Hôpital d'enfants, Groupe hospitalier de la Timone, Marseille, France
Miot, F.; Université Libre de Bruxelles - ULB
Mattei, M. G.; Hôpital d'enfants, Groupe hospitalier de la Timone, Marseille, France
Vassart, G.; Université Libre de Bruxelles - ULB
Parmentier, M.; Université Libre de Bruxelles - ULB
Language :
English
Title :
The OLFR1 gene encoding the HGMP07E putative olfactory receptor maps to the 17p12-17p13 region of the human genome and reveals a MspI restriction fragment lenght polymorphism
Bakalvar HA, Reed RR: Identification of a specialized adenylyl cyclase that may mediate odorant detection. Nature 250:1403-1406 (1990).
Baker SJ, Fearon ER, Nigro JM, Hamilton SR, Preisinger AC, Jessup JM, van Tuinen P, Ledbetter DH, Barker DF, Nakamura Y, White R, Vogelslein B: Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244:217-221 (1989).
Breer H: Molecular reaction cascades in olfactory signal transduction. J Steroid Biochem molec Biol 39:621-625 (1991).
Buck L, Axel R: A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell 65:175-187 (1991).
Dhallan RS, Yau KW, Schrader KA, Reed RR: Primary structure and functionnal expression of a cyclic nucleotide-activated channel from olfactory neurons. Nature 347:184-187 (1990).
Dohlmann HG, Thorner J, Caron MG, Lefkowitz RJ: Model systems for the study of seven transmembrane-segment receptors. A Rev Biochem 60:653-688 (1991).
Isobe M, Emanuel BS, Givol D, Oren M, Croce CM: Localization of gene for human p53 tumour antigen to band 17p13. Nature 320:84-85 (1986).
Jones DT, Reed RR: Golf: an olfactory neuron specific-G protein involved in odorant signal transduction. Science 244:790-795 (1989).
Lancet D: Vertebrate olfactory reception. A Rev Neurosci 9:329-355 (1986).
Lathrop GM, Lalouel JM: Easy calculations of lod scores and genetic risks on small computers. Amer J Hum Genet 36:460-465 (1984).
Libert F, Parmentier M, Lefort A, Dinsart C, Van Sande J, Maenhaut C, Simons MJ, Dumont JE, Vassart G: Selective amplification and cloning of four new members of the G protein-coupled receptor family. Science 244:569-572 (1989).
Mattei MG, Philip N, Passage E, Moisan JP, Mandel JL, Mattei JF: DNA probe localization at 18pl 13 band by in situ hybridization and identification of a small supernumerary chromosome. Human Genetics 69:268-271 (1985).
Nakamura T, Gold GH: A cyclic nucleotide-gated conductance in olfactory receptor cilia. Nature 325:442-444 (1987).
Pace U, Hanski E, Salomon Y, Lancet D: Odorant-sensitive adenylate cyclase of olfactory reception. Nature 316:255-258 (1985).
Parmentier M, Libert F, Maenhaut C, Lefort A, Gerard C, Perret J, Van Sande J, Dumont JE, Vassart G: Molecular cloning of the thyrotropin receptor. Science 246:1620-1622 (1989).
Parmentier M, Libert F, Schurmans S, Schiffmann S, Lefort A, Eggerickx D, Ledent C, Moltereau C, Gerard C, Perret J, Grootegoed A, Vassart G: Members of the olfactory receptor gene family are expressed in mammalian germ cells. Nature 355:453-455 (1992).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1990).
Selbie LA, Townsend-Nicholson A, Lismaa TP, Shine J: Novel G protein-coupled receptors: a gene family of putative human olfactory receptor sequences. Mol Brain Res 13:159-163 (1992).
Solomon E, Ledbetter DH: Report of the committee on the genetic constitution of chromosome 17. Eleventh International Workshop on Human Gene Mapping Cytogenet Cell Genet 58:687-738 (1991).
Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AMM, Bos JL: Genetic alterations during colorectal-tumor development. N Engl J Med 319:525-532 (1988).
