Article (Scientific journals)
Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration
Cardol, Pierre; Gloire, Geoffrey; Havaux, M. et al.
2003In Plant Physiology, 133 (4), p. 2010-2020
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Abstract :
[en] Photosynthetic activities were analyzed in Chlamydomonas reinhardtii mitochondrial mutants affected in different complexes (I, III, IV, I + III, and I + IV) of the respiratory chain. Oxygen evolution curves showed a positive relationship between the apparent yield of photosynthetic linear electron transport and the number of active proton-pumping sites in mitochondria. Although no significant alterations of the quantitative relationships between major photosynthetic complexes were found in the mutants, 77 K fluorescence spectra showed a preferential excitation of photosystem I (PSI) compared with wild type, which was indicative of a shift toward state 2. This effect was correlated with high levels of phosphorylation of light-harvesting complex II polypeptides, indicating the preferential association of light-harvesting complex II with PSI. The transition to state 1 occurred in untreated wild-type cells exposed to PSI light or in 3-(3,4-dichlorophenyl)-1,1-dimethylurea-treated cells exposed to white light. In mutants of the cytochrome pathway and in double mutants, this transition was only observed in white light in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. This suggests higher rates of non-photochemical plastoquinone reduction through the chlororespiratory pathway, which was confirmed by measurements of the complementary area above the fluorescence induction curve in dark-adapted cells. Photo-acoustic measurements of energy storage by PSI showed a stimulation of PSI-driven cyclic electron flow in the most affected mutants. The present results demonstrate that in C. reinhardtii mutants, permanent defects in the mitochondrial electron transport chain stabilize state 2, which favors cyclic over linear electron transport in the chloroplast.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Cardol, Pierre  ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale
Gloire, Geoffrey ;  Université de Liège - ULiège > Virologie - Immunologie
Havaux, M.
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Matagne, René-Fernand ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Franck, Fabrice ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie végétale
Language :
English
Title :
Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration
Publication date :
December 2003
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
Amer Soc Plant Biologists, Rockville, United States - Maryland
Volume :
133
Issue :
4
Pages :
2010-2020
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 January 2010

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