Article (Scientific journals)
Crp of Streptomyces Coelicolor Is the Third Transcription Factor of the Large Crp-Fnr Superfamily Able to Bind Camp
Derouaux, Adeline; Dehareng, Dominique; Lecocq, Elke et al.
2004In Biochemical and Biophysical Research Communications, 325 (3), p. 983-90
Peer reviewed
 

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Keywords :
cAMP-binding ability; cAMP receptor protein; germination-defective phenotype; streptomyces coelicolor
Abstract :
[en] The chromosomal inactivation of the unique transcription factor of Streptomyces coelicolor that displays a cyclic-nucleotide-binding domain, Crp(Sco), led to a germination-defective phenotype similar to the mutant of the adenylate cyclase gene (cya) unable to produce cAMP. By means of cAMP affinity chromatography we demonstrate the specific cAMP-binding ability of Crp(Sco), which definitely demonstrate that a Cya/cAMP/Crp system is used to trigger germination in S. coelicolor. However, electromobility shift assays with the purified Crp(Sco)-cAMP complex and the CRP-like cis-acting element of its own promoter failed. Moreover, we were unable to complement an Escherichia coli crp mutant in trans with Crp(Sco). The fact that Vfr from Pseudomonas aeruginosa and GlxR from Corynebacterium glutamicum could complement such an E. coli mutant suggests that the way Crp(Sco) interacts with DNA should mechanistically differ from its most similar members. This hypothesis was further supported by homology modelling of Crp(Sco) that confirmed an unusual organisation of the DNA-binding domain compared to the situation observed in Crp(Eco).
Disciplines :
Microbiology
Biotechnology
Genetics & genetic processes
Author, co-author :
Derouaux, Adeline ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Dehareng, Dominique ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Lecocq, Elke
Halici, Serkan
Nothaft, Harald
Giannotta, Fabrizio
Moutzourelis, Georgios ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Dusart, Jean;  Université de Liège - ULiège > Services administratifs généraux > R&D : Gestion opérationnelle
Devreese, Bart
Titgemeyer, Fritz
Van Beeumen, Jozef
Rigali, Sébastien  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Crp of Streptomyces Coelicolor Is the Third Transcription Factor of the Large Crp-Fnr Superfamily Able to Bind Camp
Publication date :
17 December 2004
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Elsevier, Atlanta, United States - California
Volume :
325
Issue :
3
Pages :
983-90
Peer reviewed :
Peer reviewed
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
DFG - Deutsche Forschungsgemeinschaft [DE]
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