ORBi: Derouaux Adeline - Crp of Streptomyces Coelicolor Is the Third Transcription Factor of the Large Crp-Fnr Superfamily Able to Bind Camp

Reference : Crp of Streptomyces Coelicolor Is the Third Transcription Factor of the Large Crp-Fnr Su...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology Life sciences : Genetics & genetic processes Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/3514

Title :	Crp of Streptomyces Coelicolor Is the Third Transcription Factor of the Large Crp-Fnr Superfamily Able to Bind Camp
Language :	English
Author, co-author :	Derouaux, Adeline [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
	Dehareng, Dominique [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
	Lecocq, Elke [> > > >]
	Halici, Serkan [> > > >]
	Nothaft, Harald [> > > >]
	Giannotta, Fabrizio [> > > >]
	Moutzourelis, Georgios [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
	Dusart, Jean [Université de Liège - ULg > Services administratifs généraux > R&D : Gestion opérationnelle >]
	Devreese, Bart [> > > >]
	Titgemeyer, Fritz [> > > >]
	Van Beeumen, Jozef [> > > >]
	Rigali, Sébastien [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Publication date :	17-Dec-2004
Journal title :	Biochemical and Biophysical Research Communications
Volume :	325
Issue/season :	3
Pages :	983-90
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0006-291X
Keywords :	[en] cAMP-binding ability ; cAMP receptor protein ; germination-defective phenotype
	[fr] streptomyces coelicolor
Abstract :	[en] The chromosomal inactivation of the unique transcription factor of Streptomyces coelicolor that displays a cyclic-nucleotide-binding domain, Crp(Sco), led to a germination-defective phenotype similar to the mutant of the adenylate cyclase gene (cya) unable to produce cAMP. By means of cAMP affinity chromatography we demonstrate the specific cAMP-binding ability of Crp(Sco), which definitely demonstrate that a Cya/cAMP/Crp system is used to trigger germination in S. coelicolor. However, electromobility shift assays with the purified Crp(Sco)-cAMP complex and the CRP-like cis-acting element of its own promoter failed. Moreover, we were unable to complement an Escherichia coli crp mutant in trans with Crp(Sco). The fact that Vfr from Pseudomonas aeruginosa and GlxR from Corynebacterium glutamicum could complement such an E. coli mutant suggests that the way Crp(Sco) interacts with DNA should mechanistically differ from its most similar members. This hypothesis was further supported by homology modelling of Crp(Sco) that confirmed an unusual organisation of the DNA-binding domain compared to the situation observed in Crp(Eco).
Funders :	Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Deutsche Forschungsgemeinschaft - DFG
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/3514
DOI :	10.1016/j.bbrc.2004.10.143

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