Reference : Conditional expression of type I interferon-induced bovine Mx1 GTPase in a stable tra...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/35079
Conditional expression of type I interferon-induced bovine Mx1 GTPase in a stable transgenic vero cell line interferes with replication of vesicular stomatitis virus
English
Baise, Etienne mailto [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
Pire, Grégory [Université de Liège - ULG > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies > >]
Leroy, Michael [Université de Liège - ULG > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies > >]
Gérardin, Joël mailto [Université de Liège - ULg > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies > >]
Goris, Nesya [> > > >]
De Clercq, Kris [> > > >]
Kerkhofs, Pierre [> > > >]
Desmecht, Daniel mailto [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
2004
Journal of Interferon & Cytokine Research
Mary Ann Liebert Inc
24
9
513-521
Yes (verified by ORBi)
International
1079-9907
Larchmont
[en] interferon ; Bos taurus ; Mx1
[en] In some vertebrate species, type I interferon(IFN)-induced Mx gene expression has been shown to confer resistance to some single-stranded RNA (ssRNA) viruses in vitro. Because the bovine species is subject to an exceptionally wide array of infections caused by such viruses, it is anticipated that an antiviral allele should have been retained by evolution at the bovine Mx locus. The identification of such allele may help in evaluating the real significance of the Mx genotype for disease resistance in vivo, in deciphering host-virus molecular interactions involved, or in improving innate disease resistance of livestock through marker-assisted selection. We validated a double transgenic Vero cell clone in which the bovine Mx1 reference allele is placed under control of the human cytomegalovirus (CMV) enhancer-promoter sequence containing elements from the bacterial tetracycline resistance operon to regulate transcription. In the selected clone, transgene repression was very tight, and derepression by doxycycline led to homogeneous 48-h duration expression of physiologic levels of bovine Mx1. Expression of the transgene caused a dramatic decrease in cytopathic efficiency and a 500-5000-fold yield reduction of the Indiana and New Jersey serotypes of vesicular stomatitis virus (VSV). To our knowledge, the transgenic clone developed here is the first ever reported that allows conditional expression of an Mx protein, thus providing a valuable tool for studying functions of Mx proteins in general and that of bovine Mx1 in particular. This latter may henceforward be included in the group of Mx proteins with authenticated anti-VSV activity, which offers new research avenues into the field of host-virus interactions.
Researchers ; Professionals
http://hdl.handle.net/2268/35079

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