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Species and tissue-independent rapid regulation of aromatase activity by phosphorylations.
Charlier, Thierry; Harada, Nobuhiro; Ball, Gregory F. et al.
2009In Acta Neurologica Belgica, p. 32
 

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Abstract :
[en] Aromatase activity (AA) is rapidly inhibited in male quail brains, following expression of sexual behavior, activation of glutamatergic receptors or exposure to phosphorylating conditions. Questions remain as to whether direct aromatase phosphorylation is the common key regulatory mechanism and whether these inhibitions are specific to quail hypothalamus. We now showed that AA is rapidly downregulated in quail ovary homogenates incubated in phosphorylating conditions, similarly to what is observed in hypothalamic homogenates. To understand the processes underlying this control, we expressed human aromatase in the human cell line HEK293 and 1) researched whether human aromatase can also be rapidly modulated by phosphorylations and 2) investigated more precisely the processes involved in this rapid control of activity. AA in HEK293 was rapidly inhibited following depolarization of intact cells with 100 mM KCl or in cell lysates exposed to phosphorylating conditions. Thus inhibition of AA in phosphorylating conditions is not unique to the quail hypothalamus neural environment but seems to be a general process. We are now defining the contribution of single residues of the aromatase protein to this enzymatic control.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Charlier, Thierry ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Harada, Nobuhiro
Ball, Gregory F.
Balthazart, Jacques  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Language :
English
Title :
Species and tissue-independent rapid regulation of aromatase activity by phosphorylations.
Publication date :
2009
Event name :
8th Bi-Annual meeting of the Belgian Society for Neurosciences
Event place :
Liège, Belgium
Journal title :
Acta Neurologica Belgica
ISSN :
0300-9009
eISSN :
2240-2993
Publisher :
Acta Medica Belgica, Bruxelles, Belgium
Pages :
32
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since 08 January 2010

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