[en] Calcyphosine is a calcium binding protein discovered in the dog thyroid in 1979. Calcyphosine mRNA and immunoreactivity were detected using Western and Northern blotting in the cerebral cortex, cerebral white matter and cerebellum. Using immunohistochemistry and in situ hybridization, both are present in ependymal cells, choroid plexus cells and several types of astrocytes of the subependymal cerebral layer, the cerebellar Bergmann layer, the retinal ganglion cell layer, the optic nerve and the posterior pituitary. Both are also present in neurons of nasal olfactory mucosa, enteric Auerbach and Meissner plexuses, orthosympathic and spinal cord ganglia as well as in endocrine cells of neural crest origin in the adrenal medulla. Calcyphosine immunoreactive astrocytes were also present mainly in hemispheric cerebral gray and white matter, hemispheric subcortical structures, brain stem and spinal cord. These results show that calcyphosine is a characteristic calcium binding protein of astrocytes and ependymal cells in the central nervous system and of neurons in the peripheral nervous system. This is of interest in view of the importance of calcium regulation in these cells, and since calcyphosine a calcium binding protein phosphorylated by cAMP dependent process, may be an intermediate between cAMP and inositol phosphate cascades
Schurmans, Stéphane ; Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Institut de Biologie et de Médecine Moléculaire
Schiffmann, S. N.; Université Libre de Bruxelles - ULB
Lecocq, R.; Université Libre de Bruxelles - ULB
Conreur, J. L.; Université Libre de Bruxelles - ULB
Dumont, J. E.; Université Libre de Bruxelles - ULB
Vanderhaeghen, J. J.; Université Libre de Bruxelles - ULB
Language :
English
Title :
Calcium binding protein calcyphosin in dog central astrocytes and ependymal cells and in peripheral neurons
Abe H., Watanabe M., Yamakuni T., Kuwano R., Takahashi Y., Kondo H. Localization of gene expression of calbindin in the brain of adult rats. Neurosci. Lett. 138:1992;211-215.
Bradford M.M. A rapid and sensitive method for quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
Brookes S.J., Steele P.A., Costa M. Calretinin immunoreactivity in cholinergic motor neurones, interneurones and vasomotor neurones in the guinea-pig small intestine. Cell Tissue Res. 263:1991;471-481.
Clement S., Dumont J.E., Schurmans S. Loss of calcyphosine gene expression in mouse and other rodents. Biochem. Biophys. Res. Commun. 232:1997;407-413.
Cocchia D. Immunocytochemical localization of S-100 protein in the brain of adult rat. An ultrastructural study. Cell Tissue Res. 214:1981;529-540.
Eng L.F., Vanderhaeghen J.-J., Bignami A., Gerstl B. An acidic protein isolated from fibrous astrocytes. Brain Res. 28:1971;351-354.
Ferhat L., Charton G., Represa A., Ben-Ari Y., der-Terrossian E., Khrestchatisky M. Acidic calponin cloned from neural cells is differentially expressed during rat brain development. Eur. J. Neurosci. 8:1996;1501-1509.
Haan E.A., Boss B.D., Cowan W.M. Production and characterization of monoclonal antibodies against the brain-specific proteins 14-3-2 and S-100. Proc. Natl. Acad. Sci. USA. 79:1982;7585-7589.
Halleux, P., Conreur, J.-L., Boom, A., Schiffmann, S.N., Vanderhaeghen, J.-J., 1996. Dog calcyphosine mRNA and protein in astrocytes and ependymal cells of the central nervous system and in olfactory receptor cells and enteric neurons. Abstract book of the First Annual Meeting of the Belgian Society for Neuroscience, Brussels, May 9-10.
Harlow E., Lane D. Antibodies. A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Heizmann C.W., Hunziker W. Intracellular calcium-binding proteins: more sites than insights. Trends Biochem. Sci. 16:1991;98-103.
Hof P.R., Rosenthal R.E., Fiskum G. Distribution of neurofilament protein and calcium-binding proteins parvalbumin, calbindin, and calretinin in the canine hippocampus. J.-Chem.-Neuroanat. 11:1996;1-12.
Hof P.R., Bogaert Y.E., Rosenthal R.E., Fiskum G. Distribution of neuronal populations containing neurofilament protein and calcium-binding proteins in the canine neocortex: regional analysis and cell typology. J. Chem. Neuroanat. 11:1996;81-98.
Honda C.N. Differential distribution of calbindin-D28k and parvalbumin in somatic and visceral sensory neurons. Neuroscience. 68:1995;883-892.
Housni H.E., Radulescu A., Lecocq R., Dumont J.E., Christophe D. Cloning and sequence analysis of human calcyphosine complementary DNA. Biochem. Biophys. Acta. 1352(3):1997;249-252.
Huerta J.J., Nori S., Llamosas M.M., Vazquez M.T., Bronzetti E., Vega J.A. Calretinin immunoreactivity in human sympathetic ganglia. Anat. Embryol. Berl. 194:1996;373-378.
Ichikawa H., Jacobowitz D.M., Sugimoto T. S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat. Brain Res. 748:1997;253-257.
Junttila T., Koistinaho J., Rechardt L., Hidaka H., Okazaki K., Pelto-Huikko M. Localization of neurocalcin-like immunoreactivity in rat cranial motoneurons and spinal cord interneurons. Neurosci. Lett. 183:1995;100-103.
Kretsinger R.H. Structure and evolution of calcium-modulated proteins. CRC Crit. Rev. Biochem. 8:1980;119-174.
Lach B., Scheithauer B.W., Gregor A., Wick M.R. Colloid cyst of the third ventricle. A comparative immunohistochemical study of neuraxis cysts and choroid plexus epithelium. J. Neurosurg. 78:1993;101-111.
Lamy F.M., Lecocq R.E., Contor L.S., Dumont J.E. Carbamylcholine and thyrotropin activate distinctive pathways of protein phosphorylation in dog thyroid. Biochem. Biophys. Acta. 802:1984;301-305.
Lamy F.M., Roger P.P., Lecocq R.E., Dumont J.E. Differential protein synthesis in the induction of thyroid cell proliferation by thyrotropin, epidermal growth factor or serum. Eur. J. Biochem. 155:1986;265-272.
Lecocq R.E., Lamy F.M., Dumont J.E. Pattern of protein phosphorylation in intact stimulated cells: thyrotropin and dog thyroid. Eur. J. Biochem. 102:1979;147-152.
Lecocq R.E., Lamy F.M., Erneux C., Dumont J.E. Rapid purification and identification of calcyphosine, a calcium-binding protein phosphorylated by protein kinase A. Biochem. J. 306:1995;147-151.
Lefort A., Lecocq R.E., Libert F., Lamy F.M., Swillens S., Vassart G., Dumont J.E. Cloning and sequencing of a calcium-binding protein regulated by cyclic AMP in the thyroid. EMBO J. 8:1989;111-116.
Lefort, A., 1991. Clonage, séquençage et purification d'une protéine thyroïdienne liant le calcium et phosphorylée par un processus dépendant de l'AMP cyclique: La Calcyphosine. Mémoire présenté en vue de l'obtention du grade de docteur en Sciences Chimiques. Université libre de Bruxelles.
Mailleux P., Halleux P., Verslijpe M., Segers V., Vanderhaeghen J.-J. Neuronal localization in the rat brain of the messenger RNA encoding calcyphosine, a new calcium-binding protein. Neurosci. Lett. 123:1993;123-126.
Mattson M.P., Cheng B., Baldwin S.A., Smith-Swintosky V.L., Keller J., Geddes J.W., Scheff S.W., Christakos S. Brain injury and tumor necrosis factors induce calbindin D-28k in astrocytes: evidence for a cytoprotective response. J. Neurosci. Res. 42:1995;357-370.
McConalogue K., Low A.M., Williamson S., Bornstein J.C., Furness J.B. Calretinin-immunoreactive neurons and their projections in the guinea-pig colon. Cell Tissue Res. 276:1994;359-365.
Nemoto Y., Ikeda J., Katoh K., Koshimoto H., Yoshihara Y., Mori K. R2D5 antigen: a calcium-binding phosphoprotein predominantly expressed in olfactory receptor neurons. J. Cell Biol. 123:1993;963-976.
Okazaki K., Iino S., Inoue S., Kobayashi S., Hidaka H. Differential distribution of neurocalcin isoforms in rat spinal cord, dorsal root ganglia and muscle spindle. Biochim. Biophys. Acta. 1223:1994;311-317.
Pfeiffer B., Meyermann R., Hamprecht B. Immunohistochemical co-localization of glycogen phosphorylase with the astroglial markers glial fibrillary acidic protein and S-100 protein in rat brain sections. Histochemistry. 97:1992;405-412.
Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning, a Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Sauter A., Staudenmann W., Hughes G.H., Heizmann C.W. A novel EF-hand Ca++-binding protein from abdominal muscle of crustaceans with similarity to calcyphosine from dog thyroid. Eur. J. Biochem. 227:1995;97-101.
Schiffmann S.N., Vanderhaeghen J.J. Distribution of cells containing mRNA encoding cholecystokinin in the rat central nervous system. J. Comp. Neurol. 304:1991;219-233.
Schurmans and Vanderhaeghen, 1997. Unpublished data.
Song Z.M., Brookes S.J., Costa M. All calbindin-immunoreactive myenteric neurons project to the mucosa of the guinea-pig small intestine. Neurosci. Lett. 180:1994;219-222.
Sternberger L.A., Hardy P.H. Jr., Cuculis J.J., Meyer H. The unlabelled antibody-enzyme method of immunohistochemistry. Preparation and property of soluble antigen-antibody complex (horseradish peroxydase-antihorseradish peroxydase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18:1970;315-333.
Uehata M., Ishizaki T., Satoh H., Ono T., Kawahara T., Morishita T., Tamakawa H., Yamagami K., Inui J., Maekawa M., Narumiya S. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature. 389:1997;990-994.
