[en] n the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca2+]i). Ca2+ binding proteins are enriched in the cerebellum, but their role in information processing is not clear. Here, we show that mice deficient in calretinin (Cr-/-) are impaired in tests of motor coordination. An impairment in Ca2+ homeostasis in Cr-/- Purkinje cells was supported by the high Ca2+-saturation of calbindin-D28k in these cells. The firing behavior of Purkinje cells is severely affected in Cr-/- alert mice, with alterations of simple spike firing rate, complex spike duration, and simple spike pause. In contrast, in slices, transmission at parallel fiber- or climbing fiber-Purkinje cell synapses is unaltered, indicating that marked modifications of the firing behavior in vivo can be undetectable in slice. Thus, these results show that calretinin plays a major role at the network level in cerebellar physiology
Schiffmann, Serge N.; Université Libre de Bruxelles - ULB
Cheron, Guy; Université de Mons-Hainaut - UMH
Lohoff, Ann; Centre National de la Recherche Scientifique - CNRS > Ecole Normale Supérieure, 75005 Paris, France
D’Alcantara, Pablo; Université Libre de Bruxelles - ULB
Meyer, Michael; Université Libre de Bruxelles - ULB
Parmentier, Marc; Université Libre de Bruxelles - ULB
Schurmans, Stéphane ; Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Institut de Biologie et de Médecine Moléculaire
Language :
English
Title :
Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
Publication date :
1999
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
ISSN :
0027-8424
eISSN :
1091-6490
Publisher :
National Academy of Sciences, Washington, United States - District of Columbia
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