Reference : Genomic structure, organisation, and promoter analysis of the bovine (Bos taurus) Mx1 gene
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/35017
Genomic structure, organisation, and promoter analysis of the bovine (Bos taurus) Mx1 gene
English
Gérardin [ > > ]
Baise, Etienne mailto [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
Pire, Grégory [Université de Liège - ULG > Morphologie et Pathologie > Pathologie spéciale et autopsies > >]
Leroy, Michael [Université de Liège - ULG > Morphologie et Pathologie > Pathologie spéciale et autopsies > >]
Desmecht, Daniel mailto [Université de Liège - ULg > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies > > >]
2004
Gene
326
67-75
Yes
International
[en] interferon ; Bos taurus ; Mx1
[en] Some MX proteins are known to confer a specific resistance against a panel of single-stranded RNA viruses. Many diseases due to such viruses are known to affect cattle worldwide, raising the possibility that the identification of an antiviral isoform of a bovine MX protein would allow the implementation of genetic selection programs aimed at improving innate resistance of cattle. With this potential application in mind, the present study was designed to isolate the bovine Mx1 gene including its promoter region and to investigate its genomic organisation and promoter reactivity. The bovine Mx1 gene is made up of 15 exons. All exon-intron boundaries conformed to the consensus sequences. A PCR product that contained a approximately 1-kb, 5'-flanking region upstream from the putative transcription start site was sequenced. Unexpectedly, this DNA region did not contain TATA or CCAAT motifs. A computer scan of the region disclosed a series of putative binding sites for known cytokines and transcription factors. There was a GAAAN(1-2)GAAA(C/G) motif, typical of an interferon-sensitive responsive element, between -118 and -107 from the putative transcription start site. There were also a NF-kappaB, two interleukin-6 binding sites, two Sp1 sites and five GC-rich boxes. The region also contained 12 stretches of the GAAA type, as described in all IFN-inducible genes. Bovine Mx1 expression was assessed by Northern blotting and immunofluorescence in the Madin Darby bovine kidney cells (MDBK) cell line treated with several stimuli. In conclusion, the bovine Mx1 gene and promoter region share the major structural and functional characteristics displayed by their homologs described in the rainbow trout, chicken, mouse and man.
Researchers ; Professionals
http://hdl.handle.net/2268/35017

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Gene.pdfPublisher postprint320.09 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.