Article (Scientific journals)
Genomic structure, organisation, and promoter analysis of the bovine (Bos taurus) Mx1 gene
Gérardin; Baise, Etienne; Pire, Grégory et al.
2004In Gene, 326, p. 67-75
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Keywords :
interferon; Bos taurus; Mx1
Abstract :
[en] Some MX proteins are known to confer a specific resistance against a panel of single-stranded RNA viruses. Many diseases due to such viruses are known to affect cattle worldwide, raising the possibility that the identification of an antiviral isoform of a bovine MX protein would allow the implementation of genetic selection programs aimed at improving innate resistance of cattle. With this potential application in mind, the present study was designed to isolate the bovine Mx1 gene including its promoter region and to investigate its genomic organisation and promoter reactivity. The bovine Mx1 gene is made up of 15 exons. All exon-intron boundaries conformed to the consensus sequences. A PCR product that contained a approximately 1-kb, 5'-flanking region upstream from the putative transcription start site was sequenced. Unexpectedly, this DNA region did not contain TATA or CCAAT motifs. A computer scan of the region disclosed a series of putative binding sites for known cytokines and transcription factors. There was a GAAAN(1-2)GAAA(C/G) motif, typical of an interferon-sensitive responsive element, between -118 and -107 from the putative transcription start site. There were also a NF-kappaB, two interleukin-6 binding sites, two Sp1 sites and five GC-rich boxes. The region also contained 12 stretches of the GAAA type, as described in all IFN-inducible genes. Bovine Mx1 expression was assessed by Northern blotting and immunofluorescence in the Madin Darby bovine kidney cells (MDBK) cell line treated with several stimuli. In conclusion, the bovine Mx1 gene and promoter region share the major structural and functional characteristics displayed by their homologs described in the rainbow trout, chicken, mouse and man.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Gérardin
Baise, Etienne ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Pire, Grégory;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Leroy, Michael;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ;  Université de Liège - ULiège > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Genomic structure, organisation, and promoter analysis of the bovine (Bos taurus) Mx1 gene
Publication date :
2004
Journal title :
Gene
ISSN :
0378-1119
eISSN :
1879-0038
Publisher :
Elsevier, Netherlands
Volume :
326
Pages :
67-75
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 January 2010

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