[en] A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected
BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long
open reading frame encodes for a protein of 553 amino acids, with a calculated
molecular weight of 59,153, consisting of twelve cysteine residues and six
potential glycosylation sites. The protein sequence contains a hydrophobic region
at the N-terminus of F1 and a presumptive long transmembrane fragment near the
C-terminus. Comparison of the F proteins from NDV strains Italien and
Australia-Victoria shows that the sequences are very similar, with conservation
of most cysteine residues and of the potential glycosylation sites. The F coding
sequence was inserted into the genome of vaccinia virus under the control of
vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was
demonstrated by indirect immunofluorescence with five anti-F monoclonal
antibodies known to react with conformational epitopes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Espion, D.
Dehenau, S.
Letellier, C.
Wemers, Cd.
Brasseur, Robert ; Université Libre de Bruxelles - ULB > Laboratoire Chimie-Physique des Macromolecules aux Interfaces
Apostolov K., Sawa M.I. (1976) Enhancement of haemolysis by Newcastle disease virus (NDV) after pre-treatment with heterophile antibody and complement. J Gen Virol 33:459-469.
Blumberg B.M., Giorgi C., Rose K., Kolakofsky D. (1985) Sequence determination of the Sendai virus fusion protein gene. J Gen Virol 66:317-331.
Bratt M.A., Gallagher W.R. (1969) Preliminary analysis of the requirements for fusion from within and fusion from without by Nwcastle disease virus. Proc Natl Acad Sci USA 64:536-540.
Bosch F.X., Garten W., Klenk H-D, Rott R. (1981) Proteolytic cleavage of influenza virus hemagglutinins: primary structure of the connecting peptide between HA1 and HA2 determines proteolytic cleavability and pathogenicity of avian influenza viruses. Virology 113:725-735.
Choppin P.W., Richardson C.O., Merz D.C., Hall W.W., Scheid A. (1981) The functions and inhibition of the membrane glycoproteins of paramyxoviruses and myxoviruses and the role of the measles virus M protein in subacute sclerosing panencephalitis. J Inf Dis 143:352-363.
Collins P.L., Huang Y.T., Wertz G.W. (1984) Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proc Natl Acad Sci USA 81:7683-7687.
Collins P.L., Wertz G.W., Ball L.A., Hightower L.E. (1982) Coding assignments of the five smaller mRNAs of Newcastle disease virus. J Virol 43:1024-1031.
Elango N., Masanobre S., Coligan J.E., Norrby E., Camargo E., Venkatesan S. (1985) Respiratory syncytial virus fusion glycoprotein: Nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit. Nucleic Acids Res 13:1559-1574.
Georges J.C. (1981) Les fonctions biologiques des différents polypeptides constitutifs des paramyxovirus. Bull Inst Pasteur 79:3-78.
Gorman C. (1985) High efficiency gene transfer into mammalian cells. DNA cloning. A practical approach, vol 2 , D.M., Glover, IRL Press, Oxford Washington DC; 143-190.
Gurdon J.B., Lane C.D., Woodland H.R., Marbaix G. (1971) Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature 233:177-182.
Hamaguchi M., Yoshida T., Nishikawa K., Naruse H., Nagai Y. (1983) Transcriptive complex of Newcastle disease virus. I. Both L and P proteins are required to constitute an active complex. Virology 128:105-117.
Kingsbury D.W., Bratt M.A., Choppin P.W., Hanson R.P., Hosaka Y., ter Meulen V., Norrby E., Plouright W., Rott R., Wunner W.H. (1978) Paramyxoviridae. Intervirology 10:137-152.
Kingsbury D.W., Darlington R.W. (1968) Isolation and properties of Newcastle disease virus nucleocapsid. J Virol 2:248-255.
Kohama T., Garten W., Klenk H-D (1981) Changes in conformation and charge paralleling proteolytic activation of Newcastle disease virus glycoproteins. Virology 111:364-376.
Kozak M. (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283-292.
Krystal M., Elliott R.M., Benz E.W., Young J.F., Palese P. (1982) Evolution of influenza A and B viruses: conservation of structural features in the hemagglutinin genes. Proc Natl Acad Sci USA 79:4800-4804.
Kyte J., Doolittle R.F. (1982) A simple method for displaying the hydrophobic character of a protein. J Mol Biol 157:105-132.
Lê, Portetelle D., Ghysdael J., Gonze M., Burny A., Meulemans G. (1986) Monoclonal antibodies to hemagglutinin and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity. J Virol 57:1198-1202.
McGinnes L.W., Morrison T.G. (1986) Nucleotide sequence of the gene encoding the Newcastle disease virus fusion protein and comparisons of paramyxovirus fusion protein sequences. Virus Res 5:343-356.
Mackett M., Smith G.L., Moss B. (1985) The construction and characterization of vaccinia virus recombinants expressing the foreign genes. DNA cloning. A practical approach, vol 2 , D.M., Glover, IRL Press, Oxford Washington DC; 191-211.
Maniatis T., Fritsch E.F., Sambrook J. Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York; 1982.
Maxam A.M., Gilbert W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Meth Enzymol 65:499-460.
Merz D.C., Scheid A., Choppin P.W. (1980) Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. J Exp Med 151:275-288.
Meulemans G., Antoine O., Halen P. (1978) Préparation d'antisérums conjugués à la fluorescéine pour le diagnostic des principaux virus aviaires. Ann Méd Vét 122:45-50.
Morrison T., Ward L.J., Semerjian A. (1985) Intracellular processing of the New-castle disease virus fusion glycoprotein. J Virol 53:851-857.
Nagai Y., Klenk H-D (1977) Activation of precursor to both glycoproteins of Newcastle disease virus by proteolytic cleavage. Virology 77:125-134.
Nagai Y., Klenk H-D, Rott R. (1976) Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494-508.
Nagai Y., Ogura H., Klenk H-D (1976) Studies on the assembly of the enveloppe of Newcastle disease virus. Virology 69:523-538.
Nakada S., Creager R., Krystal M., Aaronson R.P., Palese P. (1984) Influenza C virus hemagglutinin. Comparison with influenza A and B virus hemagglutinins. J Virol 50:118-124.
Nokin P., Burny A., Cleuter Y., Huez G., Marbaix G., Chantrenne H. (1975) Isolation and characterization of highly purified globin messenger RNA from anaemic-rabbit spleen. Eur J Biochem 53:83-90.
Paterson R.G., Harris T.J.R., Lamb R.A. (1984) Fusion protein of the paramyxovirus simian virus 5: nucleotide sequence of mRNA predicts a highly hydrophobic glycoprotein. Proc Natl Acad Sci USA 81:6706-6710.
Peeples M.E., Bratt M.A. (1984) Mutation in the matrix protein of Newcastle disease virus can result in decreased fusion glycoprotein incorporation into particles and decreased infectivity. J Virol 51:81-90.
Pelham H.R.B., Jackson R.J. (1976) An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem 67:247-256.
Richardson C.D., Choppin P.W. (1983) Oligopeptides that specifically inhibit membrane fusion by paramyxoviruses: studies of the site of action. Virology 131:518-532.
Richardson C.D., Scheid A., Choppin P.W. (1980) Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the F1 or HA2 viral polypeptides. Virology 105:205-222.
Riezman H., Hase T., van Loon A.P.G.M., Grivell L.A., Suda K., Schatz G. (1983) Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane. EMBO J 2:2161-2168.
Sanger F. (1981) Determination of nucleotide sequences in DNA. Science 214:1205-1210.
Scheid A., Choppin P.W. (1973) Isolation and purification of the envelope proteins of Newcastle disease virus. J Virol 11:263-271.
Scheid A., Choppin P.W. (1977) Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology 80:54-66.
Server A.C., Smith J.A., Waxham M.N., Wolinsky J.S., Goodman H.M. (1985) Purification and amino-terminal protein sequence analysis of the mumps virus fusion protein. Virology 144:373-383.
Spriggs M.K., Olted R.A., Venkatesan S., Coligan J.E., Collins P.L. (1986) Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. Virology 152:241-251.
Topp W.C. (1981) Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113:408-411.
Udenfriend S., Kilpatrick D. (1983) Biochemistry of the enkephalins and enkephalin-containing peptides. Arch Biochem Biophys 221:309-323.
Varsanyi T.M., Jornval H., Norrby E. (1985) Isolation and characterization of the measles virus F1 polypeptide: comparison with other paramyxovirus fusion proteins. Virology 147:110-117.
Von Heijne G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14:4683-4690.
Winter G., Fields S., Brownlee G.G. (1981) Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype. Nature 292:72-75.
