Reference : Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/34893
Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna
English
Espion, D. [> > > >]
Dehenau, S. [> > > >]
Letellier, C. [> > > >]
Wemers, Cd. [> > > >]
Brasseur, Robert mailto [Université Libre de Bruxelles - ULB > > Laboratoire Chimie-Physique des Macromolecules aux Interfaces > >]
Young, Jf. [> > > >]
Gross, M. [> > > >]
Rosenberg, M. [> > > >]
Meulemans, G. [> > > >]
Burny, A. [> > > >]
1987
Archives of Virology
95
1-2
79-95
Yes (verified by ORBi)
International
0304-8608
[en] A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected
BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long
open reading frame encodes for a protein of 553 amino acids, with a calculated
molecular weight of 59,153, consisting of twelve cysteine residues and six
potential glycosylation sites. The protein sequence contains a hydrophobic region
at the N-terminus of F1 and a presumptive long transmembrane fragment near the
C-terminus. Comparison of the F proteins from NDV strains Italien and
Australia-Victoria shows that the sequences are very similar, with conservation
of most cysteine residues and of the potential glycosylation sites. The F coding
sequence was inserted into the genome of vaccinia virus under the control of
vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was
demonstrated by indirect immunofluorescence with five anti-F monoclonal
antibodies known to react with conformational epitopes.
Researchers ; Professionals
http://hdl.handle.net/2268/34893
http://www.ncbi.nlm.nih.gov/pubmed/3592986

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