ORBi: Boulenouar S. - Effects of HPV-16 E5, E6 and E7 Proteins on Survival, Adhesion, Migration and Invasion of Trophoblastic Cells

Reference : Effects of HPV-16 E5, E6 and E7 Proteins on Survival, Adhesion, Migration and Invasion o...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/34887

Title :	Effects of HPV-16 E5, E6 and E7 Proteins on Survival, Adhesion, Migration and Invasion of Trophoblastic Cells
Language :	English
Author, co-author :	Boulenouar, S. [ > > ]
	Weyn, C. [ > > ]
	Van Noppen, M. [ > > ]
	Moussa Ali, M. [ > > ]
	Favre, M. [ > > ]
	Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Bex, F. [ > > ]
	Noël, Agnès [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
	Englert, Y. [ > > ]
	Fontaine, V. [ > > ]
Publication date :	2010
Journal title :	Carcinogenesis
Publisher :	Irl Press at Oxford University Press
Volume :	31
Issue/season :	3
Pages :	473-80
Audience :	International
ISSN :	0143-3334
e-ISSN :	1460-2180
City :	Oxford
Country :	United Kingdom
Abstract :	[en] Amongst high-risk human papillomaviruses (HPV), HPV-16 infection is the most prevalent causative factor for cervical cancer. Beside other mucosal targets, HPV-16 was reported to infect the placenta and to replicate in trophoblastic cells. Since these cells share invasive properties of tumoral cells, they represent an ideal model to investigate several oncogenic processes. In the present work, we analyzed the impacts of HPV-16 E5, E6 and E7 oncoproteins on the trophoblastic model. Our results showed that E5 impaired the viability of trophoblastic and cervical cell lines but E6 and E7, favouring cell growth, neutralised the E5 cytotoxic effect. In addition, E5 decreased the adhesiveness of trophoblastic cells to the tissue culture plastic and to endometrial cells similarly as previously described for E6 and E7. E5 and E6 plus E7 increased also their migration and their invasive properties. Cells expressing HPV-16 early proteins under the control of the LCR endogenous promoter displayed growth advantage and were also more motile and invasive compared to control cells. Interestingly, the E-cadherin was down regulated in trophoblastic cells expressing E5, E6 and E7. NF-kB and AP-1 activities were also enhanced. In conclusion, HPV-16 early proteins enhanced trophoblastic growth and intensify the malignant phenotype by impairing cell adhesion leading to increased cellular motile and invasive properties. HPV-16 E5 participated, with E6 and E7, in these changes by impairing Ecadherin expression, a hallmark of malignant progression.
Permalink :	http://hdl.handle.net/2268/34887
DOI :	10.1093/carcin/bgp281

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Open access	Boulenouar S Carc 2009.pdf		Author postprint	4.81 MB	View/Open

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