Article (Scientific journals)
Characterization of an antibody panel for immunohistochemical analysis of canine muscle cells
Gofflot, Stéphanie; Kischel, Philippe; Thielen, Caroline et al.
2008In Veterinary Immunology and Immunopathology, 125 (3-4), p. 225-33
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Keywords :
muscle markers; immunohistochemistry; canine muscle
Abstract :
[en] Immunohistochemistry is an indispensable tool in the assessment and characterization of lineage-specific differentiation of grafted cells in cell-based-therapy. This strategy is under investigation for the treatment of many muscle disorders and different animals such as dogs are used as models to study the tissue regeneration. The aim of the present study was to characterize an antibody panel for the analysis of canine muscle cells, useful in routinely processed formalin-fixed paraffin-embedded tissues. Overall, 12 antibodies (8 mouse monoclonal and 4 goat polyclonal), validated for use on human tissues tested for cross-reactivity on canine smooth muscle (bladder, intestine, and uterus), skeletal muscle and heart. Specific staining was achieved with eight antibodies, of which six were cytoplasmic markers (desmin, HDAC8, MHC, SMA, Troponin I and Troponin T) and two were cardiac nuclear markers (GATA-4 and Nkx-2.5). This antibody panel may be useful not only for the evaluation of cell-based therapies in muscle disorders, but also for the evaluation of canine soft tissue neoplasms in veterinary pathology.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Gofflot, Stéphanie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Kischel, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Thielen, Caroline ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Radermacher, Vincent ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Boniver, Jacques ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
de Leval, Laurence ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques - Département des sciences biomédicales et précliniques
Language :
English
Title :
Characterization of an antibody panel for immunohistochemical analysis of canine muscle cells
Publication date :
15 October 2008
Journal title :
Veterinary Immunology and Immunopathology
ISSN :
0165-2427
eISSN :
1873-2534
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
125
Issue :
3-4
Pages :
225-33
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 January 2009

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