ORBi: Gofflot Stéphanie - Immunoquantitative PCR for prion protein detection in sporadic Creutzfeldt-Jakob disease.

Reference : Immunoquantitative PCR for prion protein detection in sporadic Creutzfeldt-Jakob disease.


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Laboratory medicine & medical technology Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Anatomy (cytology, histology, embryology...) & physiology
	To cite this reference:	http://hdl.handle.net/2268/3465

Title :	Immunoquantitative PCR for prion protein detection in sporadic Creutzfeldt-Jakob disease.
Language :	English
Author, co-author :	Gofflot, Stéphanie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Deprez, Manuel [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Elmoualij, Benaïssa [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Osman, Awad [> > > >]
	Thonnart, Jean-François [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Hougrand, Olivier [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
	Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Zorzi, Willy [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques >]
Publication date :	2005
Journal title :	Clinical Chemistry
Publisher :	American Association for Clinical Chemistry
Volume :	51
Issue/season :	9
Pages :	1605-11
Audience :	International
ISSN :	0009-9147
e-ISSN :	1530-8561
City :	Washington
Country :	DC
Keywords :	[en] Adolescent ; Aged ; Aged, 80 and over ; Blotting, Western ; Brain Chemistry ; Creutzfeldt-Jakob Syndrome/diagnosis ; Endopeptidase K/metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Polymerase Chain Reaction ; Prions/analysis/metabolism ; Sensitivity and Specificity
Abstract :	[en] BACKGROUND: The most common human prion disorder is Creutzfeldt-Jakob disease (CJD); it includes sporadic, familial, iatrogenic, and variant subtypes. Diagnostic tests aim at detection with the highest specificity of very small deposits of abnormal prion protein (PrP). METHODS: We used immunoquantitative PCR (iqPCR) to detect proteinase K-resistant PrP (PrPRes) in tissue from the middle frontal gyrus of 7 patients with sporadic CJD and 7 non-CJD cases. We compared iqPCR with routine optimized ELISA, Western blotting, and immunohistochemical analyses. RESULTS: The 4 methods showed similar 100% sensitivity and specificity for the diagnosis of CJD. Along with high specificity, however, iqPCR had a threshold for PrP(Res) detection at least 10-fold lower than that of the classic ELISA. CONCLUSIONS: iqPCR is a new method for PrPRes detection that combines 100% specificity with a detection threshold at least 10-fold lower than classic techniques. This method may improve the detection of minute PrPRes deposits in tissues and body fluids and thus be useful for diagnostic and sterilization applications.
Permalink :	http://hdl.handle.net/2268/3465
	also: http://hdl.handle.net/2268/26238
DOI :	10.1373/clinchem.2005.050120

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