Reference : Immunoquantitative PCR for prion protein detection in sporadic Creutzfeldt-Jakob disease.
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/3465
Immunoquantitative PCR for prion protein detection in sporadic Creutzfeldt-Jakob disease.
English
Gofflot, Stéphanie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Deprez, Manuel [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Elmoualij, Benaïssa mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Osman, Awad [> > > >]
Thonnart, Jean-François mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Hougrand, Olivier mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques >]
2005
Clinical Chemistry
American Association for Clinical Chemistry
51
9
1605-11
Yes (verified by ORBi)
International
0009-9147
1530-8561
Washington
DC
[en] Adolescent ; Aged ; Aged, 80 and over ; Blotting, Western ; Brain Chemistry ; Creutzfeldt-Jakob Syndrome/diagnosis ; Endopeptidase K/metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Polymerase Chain Reaction ; Prions/analysis/metabolism ; Sensitivity and Specificity
[en] BACKGROUND: The most common human prion disorder is Creutzfeldt-Jakob disease (CJD); it includes sporadic, familial, iatrogenic, and variant subtypes. Diagnostic tests aim at detection with the highest specificity of very small deposits of abnormal prion protein (PrP). METHODS: We used immunoquantitative PCR (iqPCR) to detect proteinase K-resistant PrP (PrPRes) in tissue from the middle frontal gyrus of 7 patients with sporadic CJD and 7 non-CJD cases. We compared iqPCR with routine optimized ELISA, Western blotting, and immunohistochemical analyses. RESULTS: The 4 methods showed similar 100% sensitivity and specificity for the diagnosis of CJD. Along with high specificity, however, iqPCR had a threshold for PrP(Res) detection at least 10-fold lower than that of the classic ELISA. CONCLUSIONS: iqPCR is a new method for PrPRes detection that combines 100% specificity with a detection threshold at least 10-fold lower than classic techniques. This method may improve the detection of minute PrPRes deposits in tissues and body fluids and thus be useful for diagnostic and sterilization applications.
http://hdl.handle.net/2268/3465
also: http://hdl.handle.net/2268/26238
10.1373/clinchem.2005.050120

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