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Optimisation of Panax ginseng liquid cell cultures for biomass accumulation and ginsenoside production.
Kevers, Claire; Bare, Gislain; Gaspar, Thomas et al.
2005In HVOSLEF-EIDE, Anne Kathrine; PREIL, Walter (Eds.) Liquid Culture Systems for in vitro Plant Propagation
 

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Keywords :
Auxin; benzo[b]selenienyl acetic acid; biomass; cytokinin; ginseng; inoculum size; medium composition
Abstract :
[en] Solid calli and derived liquid cell cultures were initiated from one-year-old roots of Panax ginseng CA Meyer. Half-strength Murashige and Skoog medium supplemented classically with an auxin and a cytokinin did not appear favourable for biomass accumulation nor for a high ginsenoside content. Changes in the levels of mineral nutrients, sucrose and growth regulators were preliminary investigated here to improve growth and ginsenoside production in liquid cultures. The hypothesis that ginseng cells released growth inhibitors in the medium was not supported by the results obtained in experiments involving frequent transfers to fresh growth medium
Disciplines :
Biotechnology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Kevers, Claire ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Bare, Gislain;  Université de Liège - ULiège > CWBI
Gaspar, Thomas ;  Botanique, radiobotanique & serres expér.
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Dommes, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Language :
English
Title :
Optimisation of Panax ginseng liquid cell cultures for biomass accumulation and ginsenoside production.
Publication date :
2005
Main work title :
Liquid Culture Systems for in vitro Plant Propagation
Editor :
HVOSLEF-EIDE, Anne Kathrine
PREIL, Walter
Publisher :
Springer
ISBN/EAN :
978-1-4020-3199-1
Pages :
547-555
Name of the research project :
COST 848.10
Available on ORBi :
since 05 January 2009

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