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Construction of a bovine genomic library of large yeast artificial chromosome clones
Libert, F.; Lefort, A.; Okimoto, R. et al.
1993In Genomics, 18 (2), p. 270-276
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Keywords :
Bovine; Chromosome DNA; Molecular cloning; Artificial chromosome; Gene library; Artiodactyla
Abstract :
[en] We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8×12 positions) and in 2×224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Libert, F.
Lefort, A.
Okimoto, R.
Georges, Michel  ;  Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Language :
English
Title :
Construction of a bovine genomic library of large yeast artificial chromosome clones
Publication date :
1993
Journal title :
Genomics
ISSN :
0888-7543
eISSN :
1089-8646
Publisher :
Academic Press
Volume :
18
Issue :
2
Pages :
270-276
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 January 2010

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