[en] We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8×12 positions) and in 2×224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Libert, F.
Lefort, A.
Okimoto, R.
Georges, Michel ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Language :
English
Title :
Construction of a bovine genomic library of large yeast artificial chromosome clones
Albertsen, H. M., Le Paslier, D., Abderrahim, H., Dausset, J., Cann, H., and Cohen, D. (1989). Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg++. Nucleic Acids Res. 17: 808.
Albertsen, H. M., Abderrahim, H., Cann, H. M., Dausset, J., Le Pas-lier, D., and Cohen, D. (1990). Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. Proc. Natl. Acad. Sei. USA 87: 4256-4260.
Alexander, L. J., Stewart, A. F., Mackinlay, A. G., Kapelinskaya, T. V., Tkach, T. M., and Gorodetsky, S. I. (1988). Isolation and characterization of the bovine kappa-casein gene. Eur. J. Biochem. 178: 395-401.
Anand, R., Riley, J. H., Butler, R., Smith, J. C., and Markham, A. F. (1990). A 3.5 genome equivalent multi access YAC library: Construction, characterization, screening and storage. Nucleic Acids Res. 18: 1951-1956.
Anand, R., Villasante, A., and Tyler-Smith, C. (1989). Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulse-field gel electrophoresis. Nucleic Acids Res. 17: 3425-3433.
Barillot, E., Lacroix, B., and Cohen, D. (1991). Theoretical analysis of library screening using a N-dimensional pooling strategy. Nucleic Acids Res. 19: 6241-6247.
Bellanne-Chantelot, C., Lacroix, B., Ougen, P., Billault, A., Beaufils, S., Bertrand, S., Georges, I., Glibert, F., Gros, I., Lucotte, G., Susini, L., Codani, J.-J., Gesnouin, P., Pook, S., Vaysseix, G., Lu-Kuo, J., Ried, T., Ward, D., Chumakov, I., Le Paslier, D., Barillot, E., and Cohen, D. (1992). Mapping the whole human genome by fingerprinting yeast artificial chromosomes. Cell 70: 1059-1068.
Brownstein, B. H., Silverman, G. A., Little, R. D., Burke, D. T., Kors-meyer, S. J., Schlessinger, D., and Olson, M. V. (1989). Isolation of single-copy human genes from a library of yeast artificial chromosome clones. Science 244: 1348-1351.
Burgers, P. M. J., and Percival, K. J. (1987). Transformation of yeast spheroplasts without cell fusion. Anal. Biochem. 163: 391-397.
Burke, D. T., Carle, G. F., and Olson, M. V. (1987). Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812.
Connelly, C., Mc Cormick, M. K., Shero, J., and Hieter, P. (1991). Polyamines eliminate an extreme size bias against transformation of large yeast artificial chromosome DNA. Genomics 10: 10-16.
Coulson, A., Waterston, R., Kiff, J., and Kohara, Y. (1988). Genomic linking with yeast artificial chromosomes. Nature 335: 184-186.
Fries, R., Beckmann, J. S., Georges, M., Söller, M., and Womack, J. (1989). The bovine gene map. Amm. Genet. 20: 3-29.
Garza, D., Ajioka, W., Burke, D. T., and Hartl, D. L. (1989). Mapping the Drosophila genome with yeast artificial chromosome. Science 246: 641-646.
Georges, M., Dietz, A. B., Mishra, A., Nielsen, D., Sargeant, L. S., Sorensen, A., Steele, M. R., Zhao, X., Leipold, H., Womack, J. E., and Lathrop, M. (1993a). Microsatellite mapping of the gene causing weaver disease in cattle will allow the study of an associated quantitative trait locus. Proc. Natl. Acad. Sei. USA, 90: 1058-1062.
Georges, M., Drinkwater, R., King, T., Mishra, A., Moore, S. S., Nielsen, D., Sargeant, L. S., Sorensen, A., Steele, M. R., Zhao, X., Womack, J., and Hetzel, J. (1993b). Microsatellite mapping of a gene affecting horn development in Bos taurus. Nature Genet., 3: 206-210.
Green, E. D., and Olson, M. V. (1990a). Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction. Proc. Natl. Acad. Sei. USA 87: 1213-1217.
Green, E. D., and Olson, M. V. (1990b). Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: A model for human genome mapping. Science 250: 94-98.
Grosclaude, F. (1988). Le polymorphisme genetique des principales lactoproteines bovines. INRA Prod. Anim. 1: 5-17.
Hanset, R. (1982). Major genes in animal production, examples and perspectives: Cattle and pigs. Proc. 2nd World Cong. Genet. Appl. Livestock Prod. 6: 439-453.
Hino, O., Testa, J. R., Buetow, K. H., Taguchi, T., Zhou, J., Bremer, M., Bruzel, A., Yeung, R., Levan, G., Levan, K. K., Knudson, A. G., and Tartof, K. D. (1993). Universal mapping probes and the origin of human chromosome 3. Proc. Natl. Acad. Sei. USA 90: 730-734.
Imai, T., and Olson, M. V. (1990). Second-generation approach to the construction of yeast artificial chromosome libraries. Genomics 8: 297-303.
Kere, J., Nagaraja, R., Mumm, S., Ciccodicola, A., D’Urso, M., and Schlessinger, D. (1992). Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts. Genomics 14: 241-248.
Koczan, D., Hobom, G., and Seyfert, H.-M. (1991). Genomic organization of the bovine α-SI casein gene. Nucleic Acids Res. 19: 5591-5596.
Larin, Z., Monaco, A. P., and Lehrach, H. (1991). Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc. Natl. Acad. Sei. USA 88: 4123-4127.
McCormick, M. K., Shero, J. H., Cheung, M. C., Kan, Y. W., Hieter, P. A., and Antonarakis, S. E. (1989). Construction of human chromosome 21-specific yeast artificial chromosomes. Proc. Natl. Acad. Sei. USA 86: 9991-9995.
Neibergs, H. L., Gallagher, D. S., Georges, M., and Womack, J. E. (1993). Physical mapping of Inhibin beta-A in domestic cattle. Submitted for publication.
O’Brien, S. J. (1991). Mammalian genome mapping: Lessons and prospects. Curr. Opin. Genet. Dev. 1: 105-111.
Olson, M. V., Hood, L., Cantor, C., and Botstein, D. (1989). A common language for physical mapping of the human genome. Science 245: 1434-1435.
Riley, J., Butler, R., Ogilvie, D., Finniear, R., Jenner, D., Powell, S., Anand, R., Smith, J. C., and Markham, A. F. (1990). A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res. 18: 2887-2890.
Rossi, J. M., Burke, D. T., Leung, J. C. M., Koos, D. S., Chen, H., and Tilghman, S. M. (1992). Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts. Proc. Natl. Acad. Sei. USA 89: 2456-2460.
Schlessinger, D., Little, R. D., Freije, D., Abidi, F., Zucchi, I., Porta, G., Pilia, G., Nagaraja, R., Johnson, S. K., Yoon, J.Y., Srivastava, A., Kere, J., Palmieri, G., Ciccodicola, A., Montanaro, V., Romano, G., Casamassini, A., and D’Urso, M. (1991). Yeast artificial chromosome-based genome mapping: Some lessons from Xq24-q28. Genomics 11: 783-793.
Swarbrick, P. A., Howes, J., and Crawford, A. M. (1992). Ovine dinucleotide repeat polymorphism at the MAF50 locus. Anim. Genet. 23: 187.
Traver, C. N., Klapholz, S., Hyman, R. W., and Davis, R. W. (1989). Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences. Proc. Natl. Acad. Sei. USA 86: 5898-5902.
Womack, J. E., and Moll, Y. D. (1986). Gene map of the cow: Conservation of linkage with mouse and man. J. Hered. 77: 2-7.