[en] Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Monte-Carlo simulation, showing that the top 5 to 10 markers could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids, and in situ hybridization demonstrate that these bovine VNTRs are scattered throughout the bovine genome, without evidence for proterminal confinement as in the human, and that at least some of them are organized as clusters. Moreover, Southern blot analysis and in situ hybridization demonstrate conservation of sequence and map location of minisatellites within Bovidae
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Georges, Michel ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Bishop, Williamson, Skolnick (1983) A model for restriction fragment length distributions. Am. J. Hum. Genet 35:795-815.
Coppietérs, Van de Weghe, Depicker, Bouquet, Van Zeveren (1990) A hypervariable pig DNA fragment. Animal Genetics 21:29-30.
Falconer Introduction to Quantitative Genetics, 3rd ed., Longman Scientific & Technical, New York; 1989.
Farber, Georges, Debock, Verhest, Simon, Verschraegen-Spae (1989) Demonstration of spontaneous XX/XY chimerism by DNA fingerprinting. Hum. Genet 182:1-3.
Fries, Hediger, Stranzinger (1986) Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization. Anim. Genet 17:287-294.
Fries, Solinas, Gunawardana (1990) Chromosomal assignment of marker loci to monitor spacing. Gene Mapping: Strategies, Techniques and Applications, L.B. Schook, H.A. Lewin, D.G. McLaren, Dekker, New York/Basel; .
Georges, Lequarré, Castelli, Hanset, Vassart (1988) DNA fingerprinting in domestic animals using four different minisatellite probes. Cytogenetic and Genome Research 47:127-131.
Georges, Cochaux, Lequarré, Young, Vassart (1988) DNA fingerprinting in man using a mouse probe related to part of the Drosophila “Per” gene. Nucleic Acids Research 15:7193.
Georges, Lathrop, Hilbert, Marcotte, Schwers, Swillens, Vassart, Hanset (1990) On the use of DNA fingerprints for linkage studies in cattle. Genomics 6:461-474.
Georges, Mishra, Sargeant, Steele, Zhao (1990) Progress towards a primary DNA marker map in cattle. Proceedings, 4th World Congress on Genetics Applied to Livestock Production, Edinburgh, July 1990 , 2nd ed.; 13:107-111.
Georges (1991) Perspectives for marker assisted selection and velogenetics in animal breeding. Applications of Research in Mammalian Development: Current Communications in Cell and Molecular Biology 4, Pedersen, McLaren, First, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; .
Georges, Massey (1991) Velogenetics or the synergistic use of marker assisted selection and germ-line manipulation. Theriogenology 35:151-156.
ISCNDA (1989) International System for Cytogenetic Nomenclature of Domestic Animals Cytogenet. Cell Genet 53:65-79.
Jeffreys, Wilson, Thein (1985) Hypervariable “minisatellites” regions in human DNA. Nature 314:67-73.
Jeffreys, Royle, Wilson, Wong (1988) Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA. Nature 332:278-280.
Jeffreys, Neumann, Wilson (1990) Repeat unit sequence variation in minisatellites A novel source of DNA polymorphism for studying variation and mutation by single molecule analysis. Cell.
Julier, De Gouyon, Georges, Guenet, Avner, Lathrop (1990) Minisatellite linkage maps in the mouse by cross-hybridization with human VNTR probes. Proceedings of the National Academy of Sciences , 2nd ed.; 87:4585-4589.
Nakamura, Leppert, O'Connell, Wolff, Holm, Culver, Martin, Fujimoto, Hoff, Kumlin, White (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235:1616-1622.
Nakamura, Julier, Wolff, Holm, O'Connell, Leppert, White (1987) Characterization of a human ‘midisatellite’ sequence. Nucleic Acids Research 15:2537-2547.
Odelberg, Plaetke, Eldridge, Ballard, O'Connell, Nakamura, Leppert, Lalouel, White (1989) Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5:915-924.
Royle, Clarkson, Wong, Jeffreys (1988) Clustering of hypervariable minisatellites in the proterminal regions of human autosomes. Genomics 3:352-360.
Smith, Simpson (1986) The use of genetic polymorphism in livestock improvement. Journal of Animal Breeding and Genetics 103:205-217.
Soller, Beckman (1982) Restriction fragment length polymorphisms and genetic improvement. Proceedings, 2nd World Congress on Genetics Applied to Livestock Production, Madrid , 2nd ed.; 6:396-404.
Steele, Georges (1991) Generation of bovine multisite haplotypes using random cosmid clones. Genomics 10:889-904.
Threadgill, Adkinson, Womack (1990) Syntenic conservation between humans and cattle. I. Human chromosome 9. Genomics 8:22-28.
Vassart, Georges, Monsieur, Brocas, Lequarre, Christophe (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235:683-684.
Womack, Moll (1986) Gene map of the cow: Conservation of linkage with mouse and man. J. Hered 77:2-7.
Wong, Wilson, Patel, Povey, Jeffreys (1987) Characterization of a panel of highly variable minisatellites cloned from human DNA. Annals of Human Genetics 51:269-288.
Wong, Wilson, Jeffreys, Thein (1986) Cloning a selected fragment from a human DNA “fingerprint”: Isolation of an extremely polymorphic minisatellite. Nucleic Acids Res 14:4605-4616.