Article (Scientific journals)
Quantitative nephelometric assay for determining myoglobin evaluated
Delanghe, J. R.; Chapelle, Jean-Paul; Vanderschueren, S. C.
1990In Clinical Chemistry, 36 (9), p. 1675-8
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Abstract :
[en] A recently introduced automated nephelometric immunoassay involving shell/core particles for determination of myoglobin (Behringwerke) was evaluated with the BNA Nephelometer. Method precision was good: the intra-assay CV varied between 1.5% and 6.1%; with daily calibration, the interassay CV ranged between 1.5% and 7.5%. For usual sample dilutions, the assay response varied linearly with myoglobin concentrations up to 23.1 nmol/L. After automatic dilution by the instrument, concentrations up to 2310 nmol/L could be measured without high-dose "hook" effect. Further manual dilution allowed measurement of myoglobin concentrations up to 26,000 nmol/L. Calibration was stable for at least seven days. We detected no significant interferences from hemoglobin, haptoglobin, bilirubin, iodine-containing contrast media, and rheumatoid factors. Treating lipemic samples with Lipoclean (Behringwerke) decreased test results. Simultaneously drawn serum and plasma samples from the same subject showed no consistent differences in myoglobin concentrations. The mean reference myoglobin concentration was 1.380 (SD 0.82) nmol/L for men and 0.878 (SD 0.45) nmol/L for women. In patients with renal insufficiency, serum creatinine values were moderately related to serum myoglobin values (r = 0.465). Although a commercial radioimmunoassay (Byk-Sangtec) and the nephelometric assay intercorrelated well (r = 0.929), values obtained by nephelometry were significantly lower (P less than 0.05). By both assays, results for heart and skeletal muscle tissue extracts showed no correlation, a finding that suggests the existence of multiple forms of myoglobin in human tissues. We conclude that immunonephelometry is a rapid, practical, and reliable method for measuring myoglobin in serum.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Delanghe, J. R.
Chapelle, Jean-Paul ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Vanderschueren, S. C.
Language :
English
Title :
Quantitative nephelometric assay for determining myoglobin evaluated
Publication date :
September 1990
Journal title :
Clinical Chemistry
ISSN :
0009-9147
eISSN :
1530-8561
Publisher :
American Association for Clinical Chemistry, Washington, United States - District of Columbia
Volume :
36
Issue :
9
Pages :
1675-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 February 2010

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