ORBi: D'Aloia Maria - The FLC-dependent vernalization pathway in Sinapis alba.

Reference : The FLC-dependent vernalization pathway in Sinapis alba.


	Document type :	Scientific congresses and symposiums : Paper published in a journal
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...)
	To cite this reference:	http://hdl.handle.net/2268/3424

Title :	The FLC-dependent vernalization pathway in Sinapis alba.
Language :	English
Author, co-author :	D'Aloia, Maria [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
	Tocquin, Pierre [Université de Liège - ULg > > Biochimie végétale >]
	Périlleux, Claire [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Publication date :	2006
Journal title :	Comparative Biochemistry and Physiology. Part A, Physiology
Publisher :	Elsevier Science
Volume :	143A
Pages :	S170
On invitation :	No
Audience :	International
ISSN :	1096-4940
City :	New York
Country :	NY
Event name :	Annual Meeting of the Society for Experimental Biology
Event date :	du 05 avril 2006 au 07 avril 2006
Event organizer :	Society for Experimental Biology
Event place (city) :	Canterbury
Event country :	United Kingdom
Keywords :	[en] Flowering ; Vernalization ; FLOWERING LOCUS C ; Sinapis alba ; Brassicaceae
Abstract :	[en] In many plants, flowering is promoted by a long exposure to cold, a process known as ‘vernalisation’. In Arabidopsis, the vernalisation pathway was shown to promote flowering via the repression of the FLOWERING LOCUS C (FLC) gene, which encodes a repressor of flowering. As far as we know, the genetical control of flowering is conserved among Brassicaceae, and we reported elsewhere cloning of flowering times genes of the photoperiodic pathway in Sinapis alba, based on sequence similarity with Arabidopsis. However, little is known about vernalisation in Sinapis. We therefore undertook a physiological and molecular study of this process. Plants of Sinapis were grown in non inductive short days and vernalised at 7°C, at the seedling stage. Vernalisation was found to accelerate flowering and an increasing effect was observed for vernalisation treatments longer than 2 weeks. We cloned an FLC-like sequence (SaFLC) by screening a cDNA library, and used it as a probe to perform expression analyses. We observed that SaFLC was almost completely repressed after 1 week of vernalisation, but repression was stable only after 2 weeks, which is consistent with the fact that 2-week is the minimal duration of vernalisation that promotes flowering. Hence the molecular mechanisms of vernalisation seem to be conserved in Sinapis and Arabidopsis.
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/3424

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