Reference : Identification of aphid salivary proteins: a proteomic investigation of Myzus persicae.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/34208
Identification of aphid salivary proteins: a proteomic investigation of Myzus persicae.
English
Harmel, Nicolas [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Letocart, E. [> > > >]
Cherqui, A. [> > > >]
Giordanengo, P. [> > > >]
Mazzucchelli, Gabriel mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Guillonneau, F. [> > > >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Haubruge, Eric mailto [Université de Liège - ULg > Services administratifs généraux > Vice-Recteur pour le site de Gembloux >]
Francis, Frédéric mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
2008
Insect Molecular Biology
Blackwell Publishing
17
2
165-74
Yes (verified by ORBi)
0962-1075
1365-2583
Oxford
United Kingdom
[en] Amylases/metabolism ; Animals ; Aphids/enzymology/metabolism ; Electrophoresis, Polyacrylamide Gel ; Glucose 1-Dehydrogenase/metabolism ; Glucose Oxidase/metabolism ; Insect Proteins/chemistry/metabolism ; NADH Dehydrogenase/metabolism ; Proteomics/methods ; Salivary Proteins and Peptides/chemistry/metabolism ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Trypsin/metabolism ; alpha-Glucosidases/metabolism
[en] The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence reactions. Here, we focused on the salivary proteome of the green peach aphid, Myzus persicae. Proteins were either directly in-solution digested or were separated by 2D SDS-PAGE before trypsin digestion. Resulting peptides were then identified by mass spectrometry coupled with database investigations. A homemade database was constituted of expressed sequence tags from the pea aphid Acyrtosiphon pisum and M. persicae. The databases were used to identify proteins related to M. persicae with a nonsequenced genome. This procedure enabled us to discover glucose oxidase, glucose dehydrogenase, NADH dehydrogenase, alpha-glucosidase and alpha-amylase in M. persicae saliva. The presence of these enzymes is discussed in terms of plant-aphid interactions.
http://hdl.handle.net/2268/34208
10.1111/j.1365-2583.2008.00790.x

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