Reference : Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31112
Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid.
English
Francis, Frédéric mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Vanhaelen, Nicolas [> > > >]
Haubruge, Eric mailto [Université de Liège - ULg > Services administratifs généraux > Vice-Recteur pour le site de Gembloux >]
2005
Archives of Insect Biochemistry and Physiology
Wiley Liss, Inc.
58
3
166-74
Yes (verified by ORBi)
International
0739-4462
1520-6327
New York
NY
[en] Adaptation, Physiological ; Analysis of Variance ; Animals ; Aphids/drug effects/enzymology ; Brassica/chemistry ; Chromatography, Affinity ; Electrophoresis, Polyacrylamide Gel ; Glutathione Transferase/metabolism ; Iodobenzenes ; Kinetics ; Plant Extracts/metabolism/pharmacology
[en] Glutathione S-transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response to secondary metabolites from Brassica plants was determined using different host plant species and confirmed using artificial diet with pure allelochemicals added. The 2,4-dinitro-1-iodobenzene (DNIB) was found to be a useful substrate for identifying particular GSTs in insects. GSTs from M. persicae were purified using different affinity chromatography columns and related kinetic parameters were calculated. GST isoenzymes were characterised using electrophoretic methods. Although SDS-PAGE results indicated similarity among the purified enzymes from each affinity column, biochemical studies indicated significant differences in kinetic parameters. Finally, the GST pattern of M. persicae was discussed in terms of insect adaptation to the presence of plant secondary substances such as the glucosinolates and the isothiocyanates, from Brassicaceae host plants.
http://hdl.handle.net/2268/31112
also: http://hdl.handle.net/2268/34196
10.1002/arch.20049

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