Article (Scientific journals)
Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form
Boucher, J. M.; Hanosset, R.; Augot, D. et al.
2005In Veterinary Parasitology, 129 (3-4), p. 259-266
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Keywords :
Echinococcus multilocularis; wild boar; polymerase chain reaction; DNA; sequence
Abstract :
[en] Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively. (c) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Veterinary medicine & animal health
Entomology & pest control
Author, co-author :
Boucher, J. M.
Hanosset, R.
Augot, D.
Bart, J. M.
Morand, M.
Piarroux, R.
Pozet-Bouhier, F.
Losson, Bertrand ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Cliquet, F.
Language :
English
Title :
Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form
Publication date :
15 May 2005
Journal title :
Veterinary Parasitology
ISSN :
0304-4017
eISSN :
1873-2550
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
129
Issue :
3-4
Pages :
259-266
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 February 2010

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