Reference : Detection of Echinococcus multilocularis in wild boars in France using PCR techniques...
Scientific journals : Article
Life sciences : Entomology & pest control
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/34169
Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form
English
Boucher, J. M. [> > > >]
Hanosset, R. [> > > >]
Augot, D. [> > > >]
Bart, J. M. [> > > >]
Morand, M. [> > > >]
Piarroux, R. [> > > >]
Pozet-Bouhier, F. [> > > >]
Losson, Bertrand mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Cliquet, F. [> > > >]
15-May-2005
Veterinary Parasitology
Elsevier Science Bv
129
3-4
259-266
Yes (verified by ORBi)
International
0304-4017
Amsterdam
[en] Echinococcus multilocularis ; wild boar ; polymerase chain reaction ; DNA ; sequence
[en] Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively. (c) 2004 Elsevier B.V. All rights reserved.
http://hdl.handle.net/2268/34169

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