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| Reference : Detection of Echinococcus multilocularis in wild boars in France using PCR techniques ag... |
| Scientific journals : Article | |||
| Life sciences : Entomology & pest control Life sciences : Phytobiology (plant sciences, forestry, mycology...) Life sciences : Veterinary medicine & animal health | |||
| http://hdl.handle.net/2268/34169 | |||
| Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form | |
| English | |
| Boucher, J. M. [> > > >] | |
| Hanosset, R. [> > > >] | |
| Augot, D. [> > > >] | |
| Bart, J. M. [> > > >] | |
| Morand, M. [> > > >] | |
| Piarroux, R. [> > > >] | |
| Pozet-Bouhier, F. [> > > >] | |
Losson, Bertrand  [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >] | |
| Cliquet, F. [> > > >] | |
| 15-May-2005 | |
| Veterinary Parasitology | |
| Elsevier Science Bv | |
| 129 | |
| 3-4 | |
| 259-266 | |
| International | |
| 0304-4017 | |
| Amsterdam | |
| [en] Echinococcus multilocularis ; wild boar ; polymerase chain reaction ; DNA ; sequence | |
| [en] Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively. (c) 2004 Elsevier B.V. All rights reserved. | |
| http://hdl.handle.net/2268/34169 |
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