Article (Scientific journals)
The Active-Site-Serine Penicillin-Recognizing Enzymes as Members of the Streptomyces R61 Dd-Peptidase Family
Joris, Bernard; Ghuysen, Jean-Marie; Dive, Georges et al.
1988In Biochemical Journal, 250 (2), p. 313-324
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Keywords :
Binding Sites; Chymotrypsin; Electricity; Models, Chemical; Quantum Theory; Subtilisins
Abstract :
[en] Homology searches and amino acid alignments, using the Streptomyces R61 DD-peptidase/penicillin-binding protein as reference, have been applied to the beta-lactamases of classes A and C, the Oxa-2 beta-lactamase (considered as the first known member of an additional class D), the low-Mr DD-peptidases/penicillin-binding proteins (protein no. 5 of Escherichia coli and Bacillus subtilis) and penicillin-binding domains of the high-Mr penicillin-binding proteins (PBP1A, PBP1B, PBP2 and PBP3 of E. coli). Though the evolutionary distance may vary considerably, all these penicillin-interactive proteins and domains appear to be members of a single superfamily of active-site-serine enzymes distinct from the classical trypsin or subtilisin families. The amino acid alignments reveal several conserved boxes that consist of strict identities or homologous amino acids. The significance of these boxes is highlighted by the known results of X-ray crystallography, chemical derivatization and site-directed-mutagenesis experiments.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Joris, Bernard ;  Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie
Dive, Georges ;  Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie
Renard, André;  Eurogentec
Dideberg, Otto;  Université de Liège - ULiège > Institut de Physique > Service de Cristallographie
Charlier, Paulette ;  Université de Liège - ULiège > Institut de Physique > Service de Cristallographie
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Kelly, Judith A.;  University of Connecticut - UCONN > Institute of Materials Science > Department of Molecular and Cell Biology
Boyington, Jeffrey;  University of Connecticut - UCONN > Institute of Materials Science > Department of Molecular and Cell Biology
Moews, Paul C.;  University of Connecticut - UCONN > Institute of Materials Science > Department of Molecular and Cell Biology
Knox, James;  University of Connecticut - UCONN > Institute of Materials Science > Department of Molecular and Cell Biology
Language :
English
Title :
The Active-Site-Serine Penicillin-Recognizing Enzymes as Members of the Streptomyces R61 Dd-Peptidase Family
Publication date :
01 March 1988
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
250
Issue :
2
Pages :
313-324
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
NIH - National Institutes of Health [US-MD]
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