Reference : Tuber formation and development of Dioscorea cayenensis-D. rotundata complex in vitro. E...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...) Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/34098

Title :	Tuber formation and development of Dioscorea cayenensis-D. rotundata complex in vitro. Effect of polyamines.
Language :	English
Author, co-author :	Ondo Ovono, Paul [Université de Liège - ULg > > > Doct. sc. (bioch., bioch. mol.&cell., bioinf.&mod.-Bologne)]
	Kevers, Claire [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
	Dommes, Jacques [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Publication date :	2009
Journal title :	In Vitro Cellular & Developmental Biology-Plant
Publisher :	Springer Science & Business Media B.V.
Audience :	International
ISSN :	1054-5476
City :	Wallingford
Country :	United Kingdom
Keywords :	[en] polyamines ; Dioscorea cayenensis-D. rotundata ; microtuberisation ; yam
Abstract :	[en] Tuberisation was obtained in vitro on yam (Dioscorea cayenensis-D.rotundata complex). The effect of exogenous polyamines on tuber formation and development (length and weight of microtubers) were investigated and discussed in relation with changes in endogenous polyamines. Application of exogenous polyamines, inhibitors of their metabolism and polyamines precursors in various concentrations positively affected microtuber formation by yam nodal cuttings and their further development. In control conditions, three weeks are needed to obtain 100% of tuberisation. With low concentrations of putrescine (10 5 or 10 6 M), tuber formation occurred earlier. Polyamine endogenous level and metabolism can be significantly affected by exogenous polyamines but modifications of endogenous free polyamines could not be directly correlated to the tuber formation process. Increases in endogenous putrescine and auxins were observed in tubers showing a better development in the presence of putrescine. These results can be used for optimising in vitro conditions for mass production of larger microtubers of the Dioscorea cayenensis-D. rotundata complex.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/34098
DOI :	10.1007/s11627-009-9256-0

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Restricted access IVPL2009-O[1].pdf Author postprint 351.86 kB Request copy

All documents in ORBi are protected by a user license.

 

© ULg Library Network |  Feedback |  ORBi legal notices

Dspace v1.5