Article (Scientific journals)
Genomic structure, promoter analysis, and expression of the porcine (Sus scrofa) Mx1 gene
Thomas, Anne; Palm, Mélanie; Broers, Aurore et al.
2006In Immunogenetics, 58, p. 383-389
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Keywords :
interferon; mx; influenza
Abstract :
[en] Allelic polymorphisms at the mouse Mx1 locus affect the probability of survival after experimental influenzal disease, raising the possibility that marker-assisted selection using the homologous locus could improve the innate resistance of pigs to natural influenza infections. Several issues need to be resolved before efficient large scale screening of the allelic polymorphism at the porcine (Sus scrofa) Mx1 locus can be implemented. First, the Mx1 genomic structure has to be established and sufficient flanking intronic sequences have to be gathered to enable simple PCR amplification of the coding portions of the gene. Then, a basic knowledge of the promoter region needs to be obtained as an allelic variation there can significantly alter absolute levels and/or tissue-specificity of MX protein expression. The results gathered here show that the porcine Mx1 gene and promoter share the major structural and functional characteristics displayed by their homologs described in cattle, mouse, chicken, and man. The crucial function of the proximal interferon-sensitive response elements motif for gene expression is also demonstrated. The sequence data compiled here will allow an extensive analysis of the polymorphisms present among the widest spectrum possible of porcine breeds with the aim to identify an Mx1 allele providing antiviral resistance.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Thomas, Anne;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale
Palm, Mélanie;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Broers, Aurore ;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Zezafoun, Hussein ;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Genomic structure, promoter analysis, and expression of the porcine (Sus scrofa) Mx1 gene
Publication date :
2006
Journal title :
Immunogenetics
ISSN :
0093-7711
eISSN :
1432-1211
Publisher :
Springer Verlag, New York, United States - New York
Volume :
58
Pages :
383-389
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 December 2009

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