Article (Scientific journals)
Varicella-zoster virus infection of adult rat sensory neurons in vitro.
Merville-Louis, M. P.; Sadzot-Delvaux, Catherine; Delree, P. et al.
1989In Journal of Virology, 63 (7), p. 3155-60
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Keywords :
Animals; Antibodies, Monoclonal; Cells, Cultured; DNA, Viral/analysis; Fluorescent Antibody Technique; Ganglia, Spinal/microbiology; Herpesvirus 3, Human/genetics/pathogenicity; Immunoenzyme Techniques; Neurons, Afferent/microbiology; RNA, Viral/analysis; Rats; Rats, Inbred Strains
Abstract :
[en] We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with VZV-infected MRC5 cells or with cell-free virus. Indirect VZV immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that VZV infection occurred selectively in neurons. VZV-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. Genome detection using cloned VZV DNA probes revealed a hybridization signal primarily with RNA. Within 1 to 6 days postinfection, a progressive increase of VZV-specific hybridization was observed in up to 50% of the neurons. RNAs corresponding to immediate-early, early, and late genes were found, and transcripts of immediate-early gene 63 were particularly abundant.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Merville-Louis, M. P.
Sadzot-Delvaux, Catherine ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique
Delree, P.
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research
Moonen, Gustave  ;  Centre Hospitalier Universitaire de Liège - CHU > Neurologie Sart Tilman
Rentier, Bernard  ;  Université de Liège - ULiège > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie
Language :
English
Title :
Varicella-zoster virus infection of adult rat sensory neurons in vitro.
Publication date :
1989
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
63
Issue :
7
Pages :
3155-60
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 December 2009

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