ORBi: Merville-Louis M. P. - Varicella-zoster virus infection of adult rat sensory neurons in vitro.

Reference : Varicella-zoster virus infection of adult rat sensory neurons in vitro.


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/33874

Title :	Varicella-zoster virus infection of adult rat sensory neurons in vitro.
Language :	English
Author, co-author :	Merville-Louis, M. P. [> > > >]
	Sadzot-Delvaux, Catherine [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique >]
	Delree, P. [> > > >]
	Piette, Jacques [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research >]
	Moonen, Gustave [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
	Rentier, Bernard [Université de Liège - ULg > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie >]
Publication date :	1989
Journal title :	Journal of Virology
Publisher :	American Society for Microbiology (ASM)
Volume :	63
Issue/season :	7
Pages :	3155-60
Audience :	International
ISSN :	0022-538X
e-ISSN :	1098-5514
City :	Washington
Country :	DC
Keywords :	[en] Animals ; Antibodies, Monoclonal ; Cells, Cultured ; DNA, Viral/analysis ; Fluorescent Antibody Technique ; Ganglia, Spinal/microbiology ; Herpesvirus 3, Human/genetics/pathogenicity ; Immunoenzyme Techniques ; Neurons, Afferent/microbiology ; RNA, Viral/analysis ; Rats ; Rats, Inbred Strains
Abstract :	[en] We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with VZV-infected MRC5 cells or with cell-free virus. Indirect VZV immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that VZV infection occurred selectively in neurons. VZV-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. Genome detection using cloned VZV DNA probes revealed a hybridization signal primarily with RNA. Within 1 to 6 days postinfection, a progressive increase of VZV-specific hybridization was observed in up to 50% of the neurons. RNAs corresponding to immediate-early, early, and late genes were found, and transcripts of immediate-early gene 63 were particularly abundant.
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/33874

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