Article (Scientific journals)
Ragged N-Termini and Other Variants of Class a Beta-Lactamases Analysed by Chromatofocusing
Matagne, André; Joris, Bernard; Van Beeumen, J. et al.
1991In Biochemical Journal, 273 (273), p. 503-10
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Abstract :
[en] Four beta-lactamases excreted by Gram-positive bacteria exhibited microheterogeneity when analysed by chromatofocusing or ion-exchange chromatography. Ragged N-termini were in part responsible for the charge variants, but deamidation of an asparagine residue was also involved, at least for the Bacillus licheniformis enzyme. The activity of a contaminating proteinase could also be demonstrated in the case of Actinomadura R39 beta-lactamase. With that enzyme, proteolysis resulted in partial inactivation, but the inactivated fragments were easily separated from the active forms. With these, as with the other enzymes, the kinetic parameters of the major variants were identical with those of the mixture within the limits of experimental error, so that the catalytic properties of these enzymes can be determined with the 'heterogeneous' preparations.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Matagne, André  ;  Université de Liège - ULiège > Laboratoire d'Enzymologie, Centre d'Ingénierie des Protéines
Joris, Bernard ;  Université de Liège - ULiège > Laboratoire d'Enzymologie, Centre d'Ingénierie des Protéines
Van Beeumen, J.;  Rijksuniversiteit Gent
Frère, Jean-Marie ;  Université de Liège - ULiège > Laboratoire d'Enzymologie, Centre d'Ingénierie des Protéines
Language :
English
Title :
Ragged N-Termini and Other Variants of Class a Beta-Lactamases Analysed by Chromatofocusing
Publication date :
01 February 1991
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, United Kingdom
Volume :
273
Issue :
273
Pages :
503-10
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 December 2009

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