Reference : Development of a procedure to simultaneously isolate RNA, DNA, and proteins from charact...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/33681
Development of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds.
English
Tchemtchoua Tateu, Victor mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Atanasova, Ganka [> > > >]
Aqil, Abdelhafid mailto [Université de Liège - ULg > Département de chimie (sciences) > Centre d'études et de rech. sur les macromolécules (CERM) >]
Maquet, Veronique [> > > >]
Jérôme, Christine mailto [Université de Liège - ULg > Département de chimie (sciences) > Centre d'études et de rech. sur les macromolécules (CERM) - Chimie des macromolécules et des matériaux organiques >]
Poumay, Yves [Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > > LABCETI > >]
Colige, Alain mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
2009
Analytical Biochemistry
Academic Press
393
1
145-7
Yes (verified by ORBi)
International
0003-2697
1096-0309
Orlando
FL
[en] Animals ; Biochemistry/methods ; Cell Line, Tumor ; Cells, Cultured ; Chitosan ; DNA/isolation & purification ; Mice ; Proteins/isolation & purification ; RNA/isolation & purification ; biomaterial ; scaffold
[en] For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype is usually limited to evaluation of cell proliferation and survival, overlooking proteomic and transcriptomic analysis. This is largely related to the lack of efficient and quantitative procedures for protein and nucleic acid purification from cells cultured on, or inside, chitosan scaffold. Here we describe an ultracentrifugation procedure enabling the simultaneous and quantitative recovery of high quality RNA, DNA and proteins from cells growing in close contact of biomaterial matrices containing chitosan.
Center for Education and Research on Macromolecules (CERM)
Researchers
http://hdl.handle.net/2268/33681
10.1016/j.ab.2009.06.013

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