Article (Scientific journals)
Interferon-induced Sus scrofa Mx1 blocks endocytic traffic of incoming influenza A virus particles
Palm, Mélanie; Garigliany, Mutien-Marie; Cornet, François et al.
2010In Veterinary Research, 41 (3), p. 29
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Keywords :
influenza; dynamin; endocytosis
Abstract :
[en] The interferon-induced Mx proteins of vertebrates are dynamin-like GTPases, some isoforms of which can additionally inhibit the life cycle of certain RNA viruses. Here we show that the porcine Mx1 protein (poMx1) inhibits replication of influenza A virus and we attempt to identify the step at which the viral life cycle is blocked. In infected cells expressing poMx1, the level of transcripts encoding the viral nucleoprotein is significantly lower than normal, even when secondary transcription is prevented by exposure to cycloheximide. This reveals that a pretranscriptional block participates to the anti-influenza activity. Binding and internalization of incoming virus particles are normal in the presence of poMx1 but centripetal traffic to the late endosomes is interrupted. Surprisingly but decisively, poMx1 significantly alters binding of early endosome autoantigen 1 to early endosomes and/or early endosome size and spatial distribution. This is compatible with impairment of traffic of the endocytic vesicles to the late endosomes.
Disciplines :
Immunology & infectious disease
Author, co-author :
Palm, Mélanie;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Garigliany, Mutien-Marie  ;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Cornet, François ;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ;  Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Interferon-induced Sus scrofa Mx1 blocks endocytic traffic of incoming influenza A virus particles
Publication date :
February 2010
Journal title :
Veterinary Research
ISSN :
0928-4249
eISSN :
1297-9716
Publisher :
EDP Sciences, Les Ulis, France
Volume :
41
Issue :
3
Pages :
29
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 December 2009

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