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| Reference : The protection of creatine kinase MM sub-bands by EDTA during storage. |
| Scientific journals : Article | |||
| Human health sciences : Laboratory medicine & medical technology | |||
| http://hdl.handle.net/2268/3327 | |||
| The protection of creatine kinase MM sub-bands by EDTA during storage. | |
| English | |
Chapelle, Jean-Paul  [Université de Liège - ULg > Département de pharmacie > Chimie médicale >] | |
| Bertrand, A. [ > > ] | |
| Heusghem, C. [> > > >] | |
| 1981 | |
| Clinica Chimica Acta | |
| Elsevier Science | |
| 115 | |
| 3 | |
| 255-62 | |
| Yes (verified by ORBi) | |
| International | |
| 0009-8981 | |
| Amsterdam | |
| The Netherlands | |
| [en] Adult ; Blood Preservation ; Creatine Kinase/blood ; Drug Stability ; Edetic Acid/pharmacology ; Female ; Humans ; Isoelectric Focusing ; Isoenzymes ; Male ; Myocardium/enzymology ; Temperature ; Time Factors | |
| [en] We examined the effect of a 5 mmol/l concentration of EDTA on the stabilization of the five serum creatine kinase MM isoenzymes, resolved by thin-layer isoelectric focusing. In patient sera, total CK and CK-MB activities were stable during storage of the samples for two months at 4 degrees C even in the absence of EDTA. However, EDTA stabilized the labile MM and MM1 sub-bands, which are the first to appear in the blood after the release from the damaged tissue and its addition to blood samples intended for determining the MM sub-band pattern is recommended. The stabilizing effect of EDTA was emphasized at higher temperatures. EDTA protected the CK-MM pattern in myocardium extracts made in normal serum and incubated at 37 degrees C during 40 h, but was unnecessary when myocardium was homogenized in heat-inactivated serum. It is thought that EDTA could act by inhibiting a heat-labile component of human serum. | |
| http://hdl.handle.net/2268/3327 |
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