Reference : Plasticity of the mitoproteome to nitrogen sources (nitrate and ammonium) in Chlamydomon...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/33115
Plasticity of the mitoproteome to nitrogen sources (nitrate and ammonium) in Chlamydomonas reinhardtii: the logic of Aox1 gene localization
English
Gérin, Stéphanie mailto [Université de Liège - ULg > > > 2e an. master bioch. & biol. moléc. & cell., fin. appr.]
Mathy, Grégory [Université de Liège - ULg > Département des sciences de la vie > Biochimie végétale >]
Blomme, Arnaud mailto [Université de Liège - ULg > > > Doct. sc. (bioch., bioch. mol.&cell., bioinf.&mod.-Bologne)]
Franck, Fabrice mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie végétale >]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
2010
Biochimica et Biophysica Acta-Bioenergetics
Elsevier Science
1797
994-1003
Yes (verified by ORBi)
International
0005-2728
Amsterdam
[en] Alternative oxidase ; nitrogen sources ; Comparative proteomics ; Mitochondria ; bioenergetics
[en] Nitrate and ammonium constitute primary inorganic nitrogen sources that can be incorporated into carbon skeletons in photosynthetic eukaryotes. In Chlamydomonas, previous studies and the present one showed that the mitochondrial AOX is up-regulated in nitrate-grown cells in comparison with ammonium-grown cells. In this work, we have performed a comparative proteomic analysis of the soluble mitochondrial proteome of Chlamydomonas cells growth either on nitrate or ammonium. Our results highlight important proteomics modifications mostly related to primary metabolism in cells grown on nitrate. We could note an up-regulation of some TCA cycle enzymes and a down-regulation of cytochrome c1 together with an up-regulation of l-arginine and purine catabolism enzymes and of ROS scavenging systems. Hence, in nitrate-grown cells, AOX may play a dual role: (1) lowering the ubiquinone pool reduction level and (2) permitting the export of mitochondrial reducing power under the form of malate for nitrate and nitrite reduction. This role of AOX in the mitochondrial plasticity makes logical the localization of Aox1 in a nitrate assimilation gene cluster.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/33115

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