Reference : Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Veterinary medicine & animal health
Human health sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/64641
Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.
English
Schneider, Nicole mailto [Université de Liège - ULg > CORD, Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux > >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Lejeune, Jean-Philippe mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Duyckaerts, Claire mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Deby-Dupont, Ginette [Centre de l'oxygène : Recherche et développement (C.O.R.D.) > > > >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
2007
Cell Biology International
Academic Press
31
878-886
Yes (verified by ORBi)
International
1065-6995
London
United Kingdom
[en] Equine ; Chondrocytes ; Oxygen ; Glucose ; Anoxia
[en] We investigated the oxygen (O2) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O2 and at glucose concentrations of 0, 1.0 or 4.5 g/L in the culture medium (n = 3). Afterwards, the O2 consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25 min. The glucose consumption and lactate release were measured at the end of the re-oxygenation period. The chondrocytes showed a minimal O2 consumption rate, which was hardly changed by anoxia. Independently from the O2 tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5 g/L glucose (n = 3). Lactate release was also independent from O2 tension, but lower for cells at 4.5 g/L glucose (n = 3). Our observations indicated that O2 consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re-oxygenation. But the chondrocytes metabolism was modified by an excess of O2 and glucose.
Centre of Oxygen Research & Development (CORD), Institut de Chimie, B6a, Sart Tilman, University of Liège, 4000 Liège, Belgium ; Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine ; Centre Europeén du Cheval, Mont-le-Soie, Vielsam, Belgium
Ministère de l’Agriculture et de la Ruralité de la Région Wallonne
Researchers ; Professionals
http://hdl.handle.net/2268/64641
also: http://hdl.handle.net/2268/33101

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
CBIauteur.pdf.pdfAuthor postprint633.23 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.