ORBi: Schneider Nicole - Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.

Reference : Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/33101

Title :	Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.
Language :	English
Author, co-author :	Schneider, Nicole [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Lejeune, Jean-Philippe [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Duyckaerts, Claire [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Sluse, Francis [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
	Deby-Dupont, Ginette [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Serteyn, Didier [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Publication date :	2007
Journal title :	Cell Biology International
Publisher :	Academic Press
Volume :	31
Pages :	878-886
Peer reviewed :	Yes (verified by ORBi)
ISSN :	1065-6995
City :	London
Country :	United Kingdom
Keywords :	[en] equine ; chondrocyte ; oxygen ; anoxia ; glucose
Abstract :	[en] We investigated the oxygen (O(2)) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O(2) and at glucose concentrations of 0, 1.0 or 4.5g/L in the culture medium (n=3). Afterwards, the O(2) consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25min. The glucose consumption and lactate release were measured at the end of the re-oxygenation period. The chondrocytes showed a minimal O(2) consumption rate, which was hardly changed by anoxia. Independently from the O(2) tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5g/L glucose (n=3). Lactate release was also independent from O(2) tension, but lower for cells at 4.5g/L glucose (n=3). Our observations indicated that O(2) consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re-oxygenation. But the chondrocytes metabolism was modified by an excess of O(2) and glucose.
Permalink :	http://hdl.handle.net/2268/33101

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