EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation.

[en] The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 +/- 0.11) decreased after anoxia/reoxygenation (1.75 +/- 0.09, p < .01), as well as state 3 and uncoupled respiration (-20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50-200 microg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 microg/ml EGb 761, they showed an increase in ADP/O (2.09 +/- 0.14, p < .05) and a decrease in state 4 respiration (-22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Du, G.

Willet, K.

Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Sluse-Goffart, Claudine; Université de Liège - ULiège

Droy-Lefaix, M.-T.

Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire

Language :

English

Title :

EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation.

Publication date :

1999

Journal title :

Free Radical Biology and Medicine

ISSN :

0891-5849

eISSN :

1873-4596

Publisher :

Elsevier Science, Tarrytown, United States - New York

Volume :

Pages :

596-604

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 18 December 2009

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Bibliography

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