Reference : Regulation of uncoupling protein activity in phosphorylating potato tuber mitochondria.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Regulation of uncoupling protein activity in phosphorylating potato tuber mitochondria.
Navet, R. [ > > ]
Douette, P. [ > > ]
Puttique-Marique, F. [ > > ]
Sluse-Goffart, C. [ > > ]
jarmuszkiewicz, W. [ > > ]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
FEBS Letters
Elsevier Science
Yes (verified by ORBi)
The Netherlands
[en] Plant mitochondria ; uncoupling protein activity ; inhibition by GTP ; Ubiquinone
[en] In isolated potato tuber mitochondria, palmitic acid (PA) can induce a H+ leak inhibited by GTP in the phosphorylating (state 3) respiration but not in the resting (state 4) respiration. The PA-induced H+ leak is constant when state 3 respiration is decreased by an inhibition of the succinate uptake with n-butyl malonate (nBM). We show that the efficiency of inhibition by GTP is decreased when state 3 respiration is progressively inhibited by antimycin A (AA) and is restored following subsequent addition of nBM. We propose that in phosphorylating potato tuber mitochondria, the redox state of ubiquinone, which can antagonistically be varied with AA and nBM, modulates inhibition of the PA-activated UCP-sustained H+ leak by GTP.

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